Effect of oral administration of diphenyl diselenide on antioxidant status, and activity of delta aminolevulinic acid dehydratase and isoforms of lactate dehydrogenase, in streptozotocin-induced diabetic rats
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Male albino rats with diabetes induced by the administration of streptozotocin (STZ) (45 mg/kg, i.v.) were treated with oral administration of diphenyl diselenide (DPDS) pre-dissolved in soya bean oil. A significant reduction in blood glucose levels was observed in STZ-induced diabetic rats treated with DPDS compared with an untreated STZ diabetic group. The pharmacological effect of DPDS was accompanied by a marked reduction in the level of glycated proteins, and restoration of the observed decreased levels of vitamin C and reduced glutathione (GSH; in liver and kidney tissues) of STZ-treated rats. DPDS also caused a marked reduction in the high levels of thiobarbituric acid reactive substances (TBARS) observed in STZ-induced diabetic group. Finally, the inhibition of catalase, delta aminolevulinic acid dehydratase (ð-ALA-D) and isoforms of lactate dehydrogenase (LDH) accompanied by hyperglycemia were prevented by DPDS in all tissues examined. Hence, in comparison with our earlier report, the present findings suggests that, irrespective of the route of administration and the delivery vehicle, DPDS can be considered as an anti-diabetic agent due to its anti-hyperglycemic and antioxidant properties.
KeywordsDelta aminolevulinic acid dehydratase Diabetes Diphenyl diselenide Lactate dehydrogenase Streptozotocin
Delta aminolevulinic acid dehydratase
Thiobarbituric acid reactive substance
I.J.K. is specifically grateful for the financial support of TWAS and CNPq. I.J.K. is a beneficiary of the TWAS-CNPq postgraduate (doctoral) fellowship program. J.B.T. gratefully acknowledges the financial support of CNPq, FAPERGS, CAPES, CAPES/SAUX and VITAE.
- Barcelo A, Rajpathak S. Incidence and prevalence of diabetes mellitus in the Americas. Pan Am J Public Health 2001;10:300–8.Google Scholar
- Berg EA, Wu JY, Campbell L, Kagey M, Stapleton SR. Insulin-like of vanadate and selenate on the expression of glucose-6-phosphate dehydrogenase and fatty acid synthase in diabetic rats. Biochemie. 1995;919–24.Google Scholar
- Kade IJ, Paixão MW, Rodrigues OED, Barbosa NBV, Braga AL, Ávila DS, et al. Comparative studies on dicholesteroyl diselenide and diphenyl diselenide as antioxidant agents and their effect on the activities of Na+/K+-ATPase and d-aminolevulinic acid dehydratase in the rat brain. Neurochem Res 2008;33:167–78.PubMedCrossRefGoogle Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:269–75.Google Scholar
- Paulmier C. Selenium reagents and intermediates. In Organic synthesis. Pergamon, Oxford; 1986.Google Scholar
- Simonoff M, Simonoff G, (eds). Le selenium et la vie. Paris: Masson; 1991.Google Scholar