Cell Biology and Toxicology

, Volume 23, Issue 2, pp 105–112 | Cite as

DA-9601 inhibits activation of the human mast cell line HMC-1 through inhibition of NF-κB

  • S. Lee
  • H.-H. Park
  • H.-Y. Son
  • J.-H. Ha
  • M.-G. Lee
  • T.-Y. Oh
  • D. H. Sohn
  • T. C. Jeong
  • S. H. Lee
  • J.-K. Son
  • S. G. Lee
  • C.-D Jun
  • S.-H. Kim
Article

Abstract

Mast cell-mediated allergic inflammation is involved in many diseases such as asthma, sinusitis, and rheumatoid arthritis. Mast cells induce synthesis and production of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 with immune regulatory properties. The formulated ethanol extract of Artemisia asiatica Nakai (DA-9601) has been reported to have antioxidative and anti-inflammatory activities. In this report, we investigated the effect of DA-9601 on the expression of pro-inflammatory cytokines by the activated human mast cell line HMC-1 and studied its possible mechanisms of action. DA-9601 dose-dependently decreased the gene expression and production of TNF-α, IL-1β, and IL-6 on phorbol 12-myristate 13-acetate (PMA)- and calcium ionophore A23187-stimulated HMC-1 cells. In addition, DA-9601 attenuated PMA- and A23187-induced activation of NF-κB as indicated by inhibition of degradation of IκBα, nuclear translocation of NF-κB, NF-κB/DNA binding, and NF-κB-dependent gene reporter assay. Our in vitro studies provide evidence that DA-9601 might contribute to the treatment of mast cell-derived allergic inflammatory diseases.

Keywords

allergic inflammation DA-9601 mast cell NF-κB pro-inflammatory cytokine 

Abbreviations

ELISA

enzyme-linked immunosorbent assay

EMSA

electrophoretic mobility shift assays

HMC

human mast cell line

IL-1β

interleukin (IL)-1β

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NF-κB

nuclear factor-κB

PBS

phosphate-buffered saline

PDTC

pyrrolidine dithiocarbamate

PMA

phorbol 12-myristate 13-acetate

RT-PCR

reverse-transcriptase polymerase chain reaction

TNF-α

tumor necrosis factor

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Copyright information

© Springer Science + Business Media, Inc 2006

Authors and Affiliations

  • S. Lee
    • 1
  • H.-H. Park
    • 1
  • H.-Y. Son
    • 1
  • J.-H. Ha
    • 1
  • M.-G. Lee
    • 1
  • T.-Y. Oh
    • 2
  • D. H. Sohn
    • 3
  • T. C. Jeong
    • 4
  • S. H. Lee
    • 4
  • J.-K. Son
    • 4
  • S. G. Lee
    • 5
  • C.-D Jun
    • 5
  • S.-H. Kim
    • 1
    • 6
  1. 1.Department of PharmacologyKyungpook National University Medical SchoolDaeguKorea
  2. 2.Dong-A Pharmaceutical Research InstituteYonginKorea
  3. 3.Department of Pharmacy, Medicinal Resources Research InstituteWonkwang UniversityIksanKorea
  4. 4.College of PharmacyYeungnam UniversityGyongsanKorea
  5. 5.Department of Life ScienceGwangju Institutive of Science and TechnologyGwangjuKorea
  6. 6.Department of Pharmacology, School of MedicineKyungpook National UniversityDaeguRepublic of Korea

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