Cell Biology and Toxicology

, Volume 22, Issue 4, pp 285–291 | Cite as

Protective effects of β-glucan extracted from Agaricus brasiliensis against chemically induced DNA damage in human lymphocytes

  • J. P. F Angeli
  • L. R. Ribeiro
  • M. L. C. Gonzaga
  • S. de A. Soares
  • M. P. S. N. Ricardo
  • M. S. Tsuboy
  • R. Stidl
  • S. Knasmueller
  • R. E. Linhares
  • M. S. Mantovani


β-Glucans (BGs) are polysaccharides that are found in the cell walls of organisms such as bacteria, fungi, and some cereals. The objective of the present study was to investigate the genotoxic and antigenotoxic effects of BG extracted from the mushroom Agaricus brasiliensis (= Agaricus blazei Murrill ss. Heinemann). The mutagenic activity of BG was tested in single-cell gel electrophoresis assays with human peripheral lymphocytes. In addition, the protective effects against the cooked food mutagen 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and (+/−)-anti-B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE), which is the main metabolite of B[a]P, and against ROS (H2O2)-induced DNA damage, were studied. The results showed that the compound itself was devoid of mutagenic activity, and that a significant dose-dependent protective effect against damage induced by hydrogen peroxide and Trp-P-2 occurred in the dose range 20–80 μg/ml. To investigate the prevention of Trp-P-2-induced DNA damage, a binding assay was carried out to determine whether BG inactivates the amine via direct binding. Since no such interactions were observed, it is likely that BG interacts with enzymes involved in the metabolism of the amine.


Agaricus brasiliensis BPDE comet assay β-glucan hydrogen peroxide Trp-P-2 



phosphate-buffered saline


Fourier transform infrared


high-performance liquid chromatography


single-cell gel electrophoresis


heterocyclic aromatic amine








reactive oxygen species


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • J. P. F Angeli
    • 1
  • L. R. Ribeiro
    • 3
  • M. L. C. Gonzaga
    • 4
  • S. de A. Soares
    • 4
  • M. P. S. N. Ricardo
    • 4
  • M. S. Tsuboy
    • 5
  • R. Stidl
    • 6
  • S. Knasmueller
    • 7
  • R. E. Linhares
    • 2
  • M. S. Mantovani
    • 1
    • 8
  1. 1.Departamento de Biologia GeralUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de MicrobiologiaUniversidade Estadual de LondrinaLondrinaBrazil
  3. 3.Programa de Pós-Graduação em Biologia Celular e Molecular, Depto. de BiologiaUNESPRio ClaroBrazil
  4. 4.Departamento de Química Orgânica e InorgânicaUniversidade Federal do CearáFortalezaBrazil
  5. 5.Departamento de Biologia GeralUNESPAssisBrazil
  6. 6.Institute of Analytical Chemistry and Food ChemistryUniversity of ViennaViennaAustria
  7. 7.Institute of Cancer ResearchMedical University of ViennaViennaAustria
  8. 8.Departamento de Biologia Geral – CCBUniversidade Estadual de Londrina – Campus UniversitárioLondrinaBrazil

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