Catalysis Letters

, Volume 145, Issue 2, pp 519–526 | Cite as

Immobilization of Carbonic Anhydrase on Modified Electrospun Poly(Lactic Acid) Membranes: Quest for Optimum Biocatalytic Performance

  • Prakash C. Sahoo
  • Nonni Soraya Sambudi
  • Seung Bin Park
  • Jay H. LeeEmail author
  • Jong-In HanEmail author


Electrospun poly (lactic acid) membranes modified with graphene oxide (GO) and nano sized metal organic frameworks (nMOF) were synthesized, characterized and used for the immobilization of carbonic anhydrase (CA). The mechanical strength and young’s modulus of the membrane were slightly decreased when GO and nMOF were introduced, due to irregularity in the dispersion. On the other hand, the incorporation of GO into the membrane increased its thermal degradation temperature. Enzyme loading was substantially improved with GO and nMOF integration (~1.83 and ~1.57 times higher than that of poly (lactic acid)). Thermal stability, storage stability and reusability of immobilized CA, all of which are important for industrial applications, were also found to be enhanced significantly.

Graphical Abstract

Improved loading, stability and biocatalytic activity of carbonic anhydrase enzyme on modified electrospun membrane are reported


Graphene oxide Nano-MOF Enzyme immobilization Electrospun PLA 



This research was supported financially by the Advanced Biomass R&D Center (ABC) (ABC-2012053875) Grant funded by the Ministry of Education, Science and Technology, Korea.

Supplementary material

10562_2014_1406_MOESM1_ESM.docx (664 kb)
Supplementary material 1 (DOCX 663 kb)


  1. 1.
    Trifonov A, Herkendell K, Tel-Vered R, Yehezkeli O, Woerner M, Willner I (2013) ACS Nano 7:11358CrossRefGoogle Scholar
  2. 2.
    Hartmann M, Kostrov X (2013) Chem Soc Rev 42:6277CrossRefGoogle Scholar
  3. 3.
    Vinoba M, Lim KS, Lee SH, Jeong SK, Alagar M (2011) Langmuir 27:6227CrossRefGoogle Scholar
  4. 4.
    Sahoo PC, Jang YN, Lee SW (2012) J Mol Catal B-Enzym 82:37CrossRefGoogle Scholar
  5. 5.
    Li J, Wang J, Gavalas VG, Atwood DA, Bachas LG (2002) Nano Lett 3:55CrossRefGoogle Scholar
  6. 6.
    Forsyth C, Yip TWS, Patwardhan SV (2013) Chem Comm 49:3191CrossRefGoogle Scholar
  7. 7.
    Wang Y, Li Z, Wang J, Li J, Lin Y (2011) Trends Biotechnol 29:205CrossRefGoogle Scholar
  8. 8.
    Keskin S, Kızılel S (2011) Ind Eng Chem Res 50:1799CrossRefGoogle Scholar
  9. 9.
    Dreyer DR, Park S, Bielawski CW, Ruoff RS (2010) Chem Soc Rev 39:228CrossRefGoogle Scholar
  10. 10.
    Jung S, Kim Y, Kim SJ, Kwon TH, Huh S, Park S (2011) Chem Comm 47:2904CrossRefGoogle Scholar
  11. 11.
    Doshi J, Reneker DH (1995) J Electrostat 35:151CrossRefGoogle Scholar
  12. 12.
    Nair S, Kim J, Crawford B, Kim SH (2007) Biomacromolecules 8:1266CrossRefGoogle Scholar
  13. 13.
    Wang ZG, Wan LS, Liu ZM, Huang XJ, Xu ZK (2009) J Mol Catal B-Enzym 56:189CrossRefGoogle Scholar
  14. 14.
    Park JM, Kim M, Lee HJ, Jang A, Min J, Kim YH (2012) Biomacromolecules 13:3780CrossRefGoogle Scholar
  15. 15.
    Nguyen ST, Nguyen HT, Rinaldi A, Nguyen NPV, Fan Z, Duong HM (2012) Colloids Surf A 414:352CrossRefGoogle Scholar
  16. 16.
    Gu ZY, Jiang JQ, Yan XP (2011) Anal Chem 83:5093CrossRefGoogle Scholar
  17. 17.
    Su XC, Sun HW, Zhou ZF, Lin HK, Chen L, Zhu SR, Chen YT (2001) Polyhedron 20:91CrossRefGoogle Scholar
  18. 18.
    Yano T, Yah WO, Yamaguchi H, Terayama Y, Nishihara M, Kobayashi M, Takahara A (2011) Polym J 43:838CrossRefGoogle Scholar
  19. 19.
    Luan Y, Zheng N, Qi Y, Tang J, Wang G (2014) Cat Sci Tech 4:925CrossRefGoogle Scholar
  20. 20.
    Tian H, Tagaya H (2007) J Mater Sci 42:3244CrossRefGoogle Scholar
  21. 21.
    Li W, Xu Z, Chen L, Shan M, Tian X, Yang C, Lv H, Qian X (2014) Chem Eng J 237:291CrossRefGoogle Scholar
  22. 22.
    Elangovan D, Yuzay IE, Selke SEM, Auras R (2012) Polym Int 61:30CrossRefGoogle Scholar
  23. 23.
    Gao J, Hu M, Li RKY (2012) J Mat Chem 22:10867CrossRefGoogle Scholar
  24. 24.
    Wong LS, Khan F, Micklefield J (2009) Chem Rev 109:4025CrossRefGoogle Scholar
  25. 25.
    Wang ZG, Wan LS, Xu ZK (2009) Soft Matter 5:4161CrossRefGoogle Scholar
  26. 26.
    Srivastava PK, Kayastha AM, Srinivasan (2001) Biotechnol Appl Biochem 34:55Google Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea
  2. 2.Department of Civil and Environmental EngineeringKorea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea

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