Banking of human ovarian tissue potentially contaminated by cancer cells: experimental model for study of cryo-stability of these cells

  • Vladimir IsachenkoEmail author
  • Xinxin Du
  • Evgenia Isachenko
  • Plamen Todorov
  • Peter Mallmann
  • Olena Shcherbak
  • Gohar Rahimi


Auto-transplantation of cryopreserved ovarian tissue for cancer survivors comes with the primary concern of the possible existence of cancer cells in the transplanting tissue. Lineal cancer cells are presented by industry in form of separated cells (suspensions). Data about experimental models for evaluation of a cryopreservation effect on viability of compacted lineal cancer cells is currently limited. This study aims to develop a suitable experimental model for cryobiological investigations of compacted cancer cells obtained after in vitro culture of a cell suspension. Suspended lineal breast cancer cells (ZR-75-1 and MDA-MB-231) were in vitro cultured in AIM V medium for formation of monolayer. Evaluation of the cell viability was performed by healing assay, transmembrane cell migration, invasion assay and immunofluorescent test of F-actin. It was established the possibility of formation of monolayer from viable cancer cells, scarification of monolayer of these cells and formation of compacted fragments. It is described also a behaviour of compacted cells during cryopreservation (saturation by permeable cryoprotectants, thawing and removal of cryoprotectants). The described method can be used for cryobiological investigations of lineal suspended cancer cells in compacted form as a model of tissues contaminated by malignant cells and solid tumors.


Cancer cells Cryopreservation Experimental model In vitro Monolayer Solid tumors 


Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Research Group for Reproductive MedicineUniversity of CologneCologneGermany
  2. 2.Maternal Hospital of Cologne UniversityCologneGermany
  3. 3.Institute of ImmunologySofiaBulgaria
  4. 4.Kharkiv State Zooveterinary AcademyKharkivUkraine

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