Cell and Tissue Banking

, Volume 20, Issue 2, pp 307–319 | Cite as

Comparison of the regeneration induced by acellular nerve allografts processed with or without chondroitinase in a rat model

  • Jin-Hyung Im
  • Joo-Yup LeeEmail author
  • Saerom Lee
  • Myung-Gyu Lee
  • Yang-Guk Chung
  • Ki-Won Kim


There have been various studies about the acellular nerve allograft (ANA) as the alternative of autologous nerve graft in the treatment of peripheral nerve defects. As well as the decellularization process methods of ANA, the various enhancement methods of regeneration of the grafted ANA were investigated. The chondroitin sulfate proteoglycans (CSPGs) inhibit the action of laminin which is important for nerve regeneration in the extracellular matrix of nerve. Chondroitinase ABC (ChABC) has been reported that it enhances the nerve regeneration by degradation of CSPGs. The present study compared the regeneration of ANA between the processed without ChABC group and the processed with ChABC group in a rat sciatic nerve 15 mm gap model. At 12 weeks postoperatively, there was not a significant difference in the histomorphometric analysis. In the functional analysis, there were no significant differences in maximum isometric tetanic force, wet muscle weight of tibialis anterior. The processed without ChABC group had better result in ankle contracture angle significantly. In conclusion, there were no significant differences in the regeneration of ANA between the processed without ChABC group and the processed with ChABC group.


Acellular nerve allograft Chondroitin sulfate proteoglycans Chondroitinase Peripheral nerve regeneration Processed nerve allograft Rat model 



The authors disclose receipt of financial and experimental support funded by Korea Public Tissue Bank (KPTB) and The Catholic University of Korea, St. Vincent’s Hospital for this research. This article was edited by a professional English language editing service, Nature Research Editing Service.


The present study was funded by Korea Public Tissue Bank and The Catholic University of Korea, St. Vincent’s Hospital.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Orthopedic SurgeryGyeongsang National University Changwon HospitalChangwonKorea
  2. 2.Department of Orthopedic SurgeryThe Catholic University of Korea College of MedicineSeoulKorea
  3. 3.Korea Public Tissue BankSeoulKorea

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