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Cell and Tissue Banking

, Volume 19, Issue 4, pp 819–826 | Cite as

Biopolymer gels as a basis of cryoprotective medium for testicular tissue of rats

  • Nataliia Volkova
  • Mariia Yukhta
  • Anatoliy Goltsev
Article
  • 20 Downloads

Abstract

Cryopreservation of testis tissue is a promising approach to save fertility in prepubertal boys under going gonadotoxic cancer therapies. The using biopolymers as a basis of cryoprotective medium can be effective for the optimization of cryopreservation protocols of immature testicular tissue. The research purpose was to determine morphological parameters and metabolic activity of seminiferous tubules of immature rat testes under exposure to cryoprotective solution (DMSO) based on collagen or fibrin gels (CG or FG) as one of the first stages of developing the cryopreservation protocol. It was found that 30-min exposure of tissue samples to CG and FG with 0.6 M DMSO did not impair the spermatogenic epithelium and metabolic activity of the cells (MTT test and total lactate dehydrogenase activity). The use of FG at the time of exposure of 45 min did not lead to significant changes in the metabolic activity in contrast to other groups. The findings could be used to substantiate and develop the effective techniques for cryopreservation of immature seminiferous tubules.

Keywords

Immature testicular tissue Spermatogenic epitelium Cryoprotective medium Collagen gel Fibrin gel Metabolic activity 

Notes

Funding

This study was carried-out within the research project of the National Academy of Sciences of Ukraine (No. 2.2.6.99).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All the manipulations with animals were carried out in accordance to the European convention for the protection of vertebrate animals used for experimental and other scientific purposes (Strasbourg, 18.III.1986). The protocols were approved by the Bioethics Committee of Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine (Permit No 2016-05).

Human and animal rights

This article does not contain any studies with human participants performed by any of the authors.

References

  1. Abrishami M, Anzar M, Yanga Y, Honaramooz A (2010) Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice. Theriogenology 73(1):86–96CrossRefPubMedGoogle Scholar
  2. Allenspach AL, Kraemer TG (1989) Ice crystal patterns in artificialgels of extracellular matrix molecules after quick-freezing and freeze-substitution. Cryobiology 26:170–179CrossRefPubMedGoogle Scholar
  3. Bret DU, Lakshmi SN, Cato TL (2011) Biomedical applications of biodegradable polymers. J Polym Sci B Polym Phys 49(12):832–864CrossRefGoogle Scholar
  4. Caires KC, de Avila J, McLean DJ (2009) Vascular endothelial growth factor regulates germ cell survival during establishment of spermatogenesis in the bovine testis. Reproduction 138(4):667–677CrossRefPubMedGoogle Scholar
  5. Chandrakasan G, Torchia DA, Piez KA (1976) Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the nonhelical ends in solution. J Biol Chem 251:6062–6067PubMedGoogle Scholar
  6. Chen B, Wright B, Sahoo R, Connon CJ (2013) A novel alternative to cryopreservation for the short-term storage of stem cells for use in cell therapy using alginate encapsulation. Tissue Eng Part C Methods 19(7):568–576CrossRefPubMedGoogle Scholar
  7. Chiti MC, Dolmans MM, Donnez J, Amorim CA (2017) Fibrin in reproductive tissue engineering: a review on its application as a biomaterial for fertility preservation. Ann Biomed Eng 45(7):1650–1663CrossRefPubMedGoogle Scholar
  8. Del Vento F, Vermeulen M, de Michele F, Giudice MG, Poels J, des Rieux A, Wyns C (2018) Tissue engineering to improve immature testicular tissue and cell transplantation outcomes: one step closer to fertility restoration for prepubertal boys exposed to gonadotoxic treatments. Int J Mol Sci 19(1):E286CrossRefPubMedGoogle Scholar
  9. Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS (2011) Polymeric scaffolds in tissue engineering application: a review. Int J Polym Sci 2011:1–19CrossRefGoogle Scholar
  10. Geckil H, Xu F, Zhang X, Moon S, Demirci U (2010) Engineering hydrogels as extracellular matrix mimics. Nanomedicine 5(3):469–484CrossRefPubMedGoogle Scholar
  11. Gelse K, Pösch E, Aigner T (2003) Collagens—structure, function, and biosynthesis. Adv Drug Deliv Rev 55(12):1531–1546CrossRefPubMedGoogle Scholar
  12. Guarino V, Ambrosio L (2014) Properties of biomedical foams for tissue engineering applications. In: Netti PA (ed) biomedical foams for tissue engineering applications. Woodhead Publishing, Cambridge, pp 40–70CrossRefGoogle Scholar
  13. Hatte L, Le Corre S, Baudot A, Louis G, Letourneur D, Doucet C, Meddahi-Pellé A (2016) New application for biohydrogels: myoblast cryopreservation for cell therapy. In: Frontiers in bioengineering and biotechnology conference abstract: 10th world biomaterials congress.  https://doi.org/10.3389/conf.FBIOE.2016.01.00444
  14. Itoh T, Kacchi M, Abe H, Hoshi H (2001) High recovery from successful cryopreservation of bovine small preantral follicles embedded within collagen gels. Tissue Cult Res Commun 21:109–119Google Scholar
  15. Jafari M, Paknejad Z, Rad MR, Motamedian SR, Eghbal MJ, Nadjmi N, Khojasteh A (2017) Polymeric scaffolds in tissue engineering: a literature review. J Biomed Mater Res B 105(2):431–459CrossRefGoogle Scholar
  16. Ji L, de Pablo JJ, Palecek SP (2004) Cryopreservation of adherent human embryonic stem cells. Biotechnol Bioeng 88(3):299–312CrossRefPubMedGoogle Scholar
  17. Keros V, Hultenby K, Borgström B, Fridstrom M, Jahnukainen K, Hovatta O (2007) Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment. Hum Reprod 22(5):1384–1395CrossRefPubMedGoogle Scholar
  18. Koebe HG, Dunn JC, Toner M, Sterling LM, Hubel A, Cravalho EG, Yarmush ML, Tompkins RG (1990) A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration. Cryobiology 27(5):576–584CrossRefPubMedGoogle Scholar
  19. Lee KW, Park JB, Yoon JJ, Lee JH, Kim SY, Jung HJ, Lee SK, Kim SJ, Lee JH, Lee DS, Joh JW (2004) The viability and function of cryopreserved hepatocyte spheroids with different cryopreservation solutions. Transpl Proc 36(8):2462–2463CrossRefGoogle Scholar
  20. Li Y, Meng H, Liu Y, Lee BP (2015) Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering. Sci World J 2015:685690Google Scholar
  21. Makarevich AV, Spalekova E, Olexikova L, Kubovicova E, Hegedusova Z (2014) Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa. Zygote 22(3):305–313CrossRefPubMedGoogle Scholar
  22. Malpique R, Osorio LM, Ferreira DS, Ehrhart F, Brito C, Zimmermann H, Alves PM (2010) Alginate encapsulation as a novel strategy for the cryopreservation of neurospheres. Tissue Eng Part C Methods 16(5):965–977CrossRefPubMedGoogle Scholar
  23. Michele F, Vermeulen M, Wyns C (2017) Fertility restoration with spermatogonial stem cells. Curr Opin Endocrinol 24(6):424–431CrossRefGoogle Scholar
  24. Milazzo JP, Vaudreuil L, Cauliez B, Gruel E, Masse L, Mousset-Simeon N, Mace B, Rives N (2008) Comparison of conditions for cryopreservation of testicular tissue from immature mice. Hum Reprod 23:17–28CrossRefPubMedGoogle Scholar
  25. Milazzo JP, Travers A, Bironneau A, Safsaf A, Gruel E, Arnoult C, Mace B, Boyer O, Rives N (2010) Rapid screening of cryopreservation protocols for murine prepubertal testicular tissue by histology and PCNA immunostaining. J Androl 31(6):617–630CrossRefPubMedGoogle Scholar
  26. Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63CrossRefGoogle Scholar
  27. Murdock MH, David S, Swinehart IT, Reing JE, Tran K, Gassei K, Orwig KE, Badylak SF (2018) Human testis extracellular matrix enhances human spermatogonial stem cell survival in vitro. Tissue Eng Part A.  https://doi.org/10.1089/ten.TEA.2018.0147 CrossRefPubMedGoogle Scholar
  28. Nair LS, Laurencin CT (2007) Biodegradable polymers as biomaterials. Progr Polym Sci 32:762–798CrossRefGoogle Scholar
  29. Nicodemus GD, Bryant SJ (2008) Cell encapsulation in biodegradable hydrogels for tissue engineering applications. Tissue Eng Part B Rewires 14:149–165CrossRefGoogle Scholar
  30. Nishiyama K, Okudera T, Watanabe T, Isobe K, Suzuki M, Masuki H, Okudera H, Uematsu K, Nakata K, Kawase T (2016) Basic characteristics of plasma rich in growth factors (PRGF): blood cell components and biological effects. Clin Exp Dent Res 2(2):96–103CrossRefPubMedPubMedCentralGoogle Scholar
  31. Saeednia S, Shabani Nashtaei M, Bahadoran H, Aleyasin A, Amidi F (2016) Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation. Reprod Biol Endocrinol 14(1):29CrossRefPubMedPubMedCentralGoogle Scholar
  32. Sakai S, Inamoto K, Liu Y, Tanaka S, Arii S, Taya M (2012) Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity. Cancer Sci 103(3):m549–m554CrossRefGoogle Scholar
  33. Swioklo S, Constantinescu A, Connon CJ (2016) Alginate-encapsulation for the improved hypothermic preservation of human adipose-derived stem cells. Stem Cell Transl Med 5(3):339–349CrossRefGoogle Scholar
  34. Takahashi T, Hirsh A, Erbe E, Williams RJ (1988) Mechanism of cryoprotection by extracellular polymeric solutes. Biophys J 54(3):509–518CrossRefPubMedPubMedCentralGoogle Scholar
  35. Tian R, Yang S, Zhu Y, Zou S, Li P, Wang J, Zhu Z, Huang Y, He Z, Li Z (2016) VEGF/VEGFR2 signaling regulates germ cell proliferation in vitro and promotes mouse testicular regeneration in vivo. Cells Tissues Organs 201(1):1–13CrossRefPubMedGoogle Scholar
  36. Travers A, Milazzo JP, Perdrix A, Metton C, Bironneau A, Mace B, Rives N (2011) Assessment of freezing procedures for rat immature testicular tissue. Theriogenology 76(6):981–990CrossRefPubMedGoogle Scholar
  37. Volkova NO, Yukhta MS, Chernyshenko LG, Stepanyuk LV, Sokol LV, Goltsev AM (2017) Exposure of seminiferous tubules of immature rats to cryoprotective media of various compositions. Probl Cryobiol Cryomed 27(3):203–218CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Cryopathophysiology and ImmunologyInstitute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of UkraineKharkivUkraine

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