Cell and Tissue Banking

, Volume 19, Issue 4, pp 799–807 | Cite as

Evaluation of linoleic acid on lipid peroxidative/antioxidative parameters, motility and viability of rooster spermatozoa during cold storage

  • Mohsen EslamiEmail author
  • Elham Zadeh Hashem
  • Abolfazl Ghaniei
  • Hossein Sayyah-Atashbeig


Sustainable reduction in semen quality due to detrimental effects of primary and secondary peroxidative products was occurred during liquid storage. The objective of the current experiment was to explore the influence of bovine serum albumin conjugated linoleic acid (LA) on the rooster spermatozoa routine tests and lipid peroxidative/antioxidative levels stored at 4 °C over 48 h. For this purpose, collected ejaculates (≥ 80% progressive motile spermatozoa) pooled and extended with the phosphate buffer medium without (control) or enriched with different amounts of LA (0.125, 0.25 or 0.50 mM). Contents of total antioxidant status (TAS) and thiobarbituric reactive substances (TBARS) were measured separately in the medium and spermatozoa, as well as percent of viability and motility at 0, 24 and 48 h intervals. Viability was not affected by treatment during the study intervals (P > 0.05). While, higher motility was recorded in LA 0.50 mM group (77.98 ± 1.89 and 57.02 ± 2.45) compared to the control group (68.78 ± 1.29 and 45.09 ± 1.86) at 24 and 48 h, respectively (P < 0.03). Amounts of TBARS in medium and spermatozoa were lower in LA 0.25 and 0.50 groups compared to the control at 48 h (P < 0.01). Moreover, TAS levels of medium and spermatozoa were lower in control samples compared to LA treated groups at 48 h (P < 0.03). Because of the ability of the LA to lowering the quantities of lipid peroxidation index and improving motility especially at 0.5 mM levels, it can be proposed as an additive during liquid storage of rooster semen.


Lipid peroxidation Linoleic acid Rooster Spermatozoa 



The authors wish to thank all personnel for their technical support during this work.


The current study was supported by the Research Deputy of the Urmia University (D10-485).

Compliance with ethical standards

The experimental procedure carried out on the roosters for semen sampling was approved by Animal Care Committee of the Urmia University.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Theriogenology, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  2. 2.Department of Basic Sciences, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  3. 3.Department of Clinical Sciences, Faculty of Veterinary MedicineFerdowsi University of MashhadMashhadIran

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