In vitro and in vivo evaluation of xenogeneic bone putty with the carrier of hydrogel derived from demineralized bone matrix
- 180 Downloads
The demineralized bone matrix (DBM) putty is a traditional bone graft utilized to facilitate the repair and reconstruction of bone. Recent studies indicated the DBM putties with the various carriers were different in bone repairing ability. In order to prepare a kind of DBM putty with a good biocompatibility and bioactivity, the DBM gel was processed from the DBM and the feasibility as a carrier for the DBM putty was evaluated. After the bovine DBM gel was prepared, the BMPs content as well as the ability to promote osteogenic differentiation of MC3T3-E1 cells in vitro were investigated. Then the DBM putty was prepared and filled into the rat calvarial defect model to evaluate the bone repairing ability by micro-CT and histology. The result showed there was 2.953 ± 0.054 ng BMP contained in per gram of the DBM gel. And the ALP production of MC3T3-E1 cells in the DBM gels group increased with prolonged culturing, the mineralized nodules formed in MC3T3-E1 cells on 14th day after co-culture. The putty prepared by DBM gel was easy to handle without loss of DBM particles at room temperature. In the rat calvarial bone defect experiment, histological observation showed more mature bone formed in the DBM putty group than that in the type I collagen group at 12 weeks, which indicated the bone putty prepared by DBM gel exhibited a better bone repair capability.
KeywordsDemineralized bone matrix Xenograft Bone matrix gel Bone regeneration Moldable putty
This work was supported by the research funding from Natural Science Foundation of Shandong Province (Nos. BS2015SW021; ZR2014HM009; ZR2016HP13), the Project of Science and Technology of Binzhou Medical University (Nos. BY2014KYQD25; BY2014KJ08), the National Natural Science Foundation of China (No. 81301570), and The development of medical science and technology project of Shandong Province (No. 2013WS0306).
Compliance with ethical standards
Conflict of interest
We declare that we have no conflicts of interest to this work, and have no financial and personal relationships with other people or organizations that can inappropriately influence our work.
- Bodakhe S, Verma S, Garkhal K, Samal SK, Sharma SS, Kumar N (2013) Injectable photocrosslinkable nanocomposite based on poly(glycerol sebacate) fumarate and hydroxyapatite: development, biocompatibility and bone regeneration in a rat calvarial bone defect model. Nanomedicine (Lond) 8(11):1777–1795CrossRefGoogle Scholar
- Borah B, Dufresne TE, Cockman MD, Gross GJ, Sod EW, Myers WR, Combs KS, Higgins RE, Pierce SA, Stevens ML (2000) Evaluation of changes in trabecular bone architecture and mechanical properties of minipig vertebrae by three-dimensional magnetic resonance microimaging and finite element modeling. J Bone Miner Res 15(9):1786–1797CrossRefPubMedGoogle Scholar
- Dozza B, Lesci IG, Duchi S, Della Bella E, Martini L, Salamanna F, Falconi M, Cinotti S, Fini M, Lucarelli E, Donati D (2017) When size matters: differences in demineralized bone matrix particles affect collagen structure, mesenchymal stem cell behavior, and osteogenic potential. J Biomed Mater Res A 105(4):1019–1033CrossRefPubMedGoogle Scholar
- Gothard D, Smith EL, Kanczler JM, Black CR, Wells JA, Roberts CA, White LJ, Qutachi O, Peto H, Rashidi H, Rojo L, Stevens MM, Haj EI, Rose FRAJ, Shakesheff KM, Oreffo RO (2015) In vivo assessment of bone regeneration in alginate/bone ECM hydrogels with incorporated skeletal stem cells and single growth factors. PLoS ONE 10(12):e0145080CrossRefPubMedPubMedCentralGoogle Scholar
- Horvathy DB, Vacz G, Toro I, Szabo T, May Z, Duarte M, Hornyak I, Szabo BT, Dobo-Nagy C, Doros A, Lacza Z (2016) Remineralization of demineralized bone matrix in critical size cranial defects in rats: a 6-month follow-up study. J Biomed Mater Res B Appl Biomater 104(7):1336–1342CrossRefPubMedGoogle Scholar
- Kim DM, Nevins M, Camelo M, Schupbach P, Kim SW, Camelo JM, Al Hezaimi K, Nevins ML (2011) The feasibility of demineralized bone matrix and cancellous bone chips in conjunction with an extracellular matrix membrane for alveolar ridge preservation: a case series. Int J Periodontics Restor Dent 31(1):39–47Google Scholar
- O’leary RK, Prewett AB (1993) Osteogenic composition and implant containing same. United States, Patent and Trademark OfficeGoogle Scholar
- O’leary RK, Prewett AB (1995) Osteogenic composition and implant containing same. United States, Patent and Trademark OfficeGoogle Scholar
- Peker E, Karaca IR, Yildirim B (2016) Experimental evaluation of the effectiveness of demineralized bone matrix and collagenated heterologous bone grafts used alone or in combination with platelet-rich fibrin on bone healing in sinus floor augmentation. Int J Oral Maxillofac Implants 31(2):e24–e31CrossRefPubMedGoogle Scholar
- Schallenberger MA, Rossmeier K, Lovick HM, Meyer TR, Aberman HM, Juda GA (2014) Comparison of the osteogenic potential of OsteoSelect demineralized bone matrix putty to NovaBone calcium-phosphosilicate synthetic putty in a cranial defect model. J Craniofac Surg 25(2):657–661CrossRefPubMedPubMedCentralGoogle Scholar