Cell and Tissue Banking

, Volume 19, Issue 4, pp 591–601 | Cite as

In vitro and in vivo evaluation of xenogeneic bone putty with the carrier of hydrogel derived from demineralized bone matrix

  • Naili Zhang
  • Lina Ma
  • Xiaowei Liu
  • Xiaorui Jiang
  • Zhenhai Yu
  • Dongmei Zhao
  • Luping Zhang
  • Chunlei Zhang
  • Fei HuangEmail author


The demineralized bone matrix (DBM) putty is a traditional bone graft utilized to facilitate the repair and reconstruction of bone. Recent studies indicated the DBM putties with the various carriers were different in bone repairing ability. In order to prepare a kind of DBM putty with a good biocompatibility and bioactivity, the DBM gel was processed from the DBM and the feasibility as a carrier for the DBM putty was evaluated. After the bovine DBM gel was prepared, the BMPs content as well as the ability to promote osteogenic differentiation of MC3T3-E1 cells in vitro were investigated. Then the DBM putty was prepared and filled into the rat calvarial defect model to evaluate the bone repairing ability by micro-CT and histology. The result showed there was 2.953 ± 0.054 ng BMP contained in per gram of the DBM gel. And the ALP production of MC3T3-E1 cells in the DBM gels group increased with prolonged culturing, the mineralized nodules formed in MC3T3-E1 cells on 14th day after co-culture. The putty prepared by DBM gel was easy to handle without loss of DBM particles at room temperature. In the rat calvarial bone defect experiment, histological observation showed more mature bone formed in the DBM putty group than that in the type I collagen group at 12 weeks, which indicated the bone putty prepared by DBM gel exhibited a better bone repair capability.


Demineralized bone matrix Xenograft Bone matrix gel Bone regeneration Moldable putty 



This work was supported by the research funding from Natural Science Foundation of Shandong Province (Nos. BS2015SW021; ZR2014HM009; ZR2016HP13), the Project of Science and Technology of Binzhou Medical University (Nos. BY2014KYQD25; BY2014KJ08), the National Natural Science Foundation of China (No. 81301570), and The development of medical science and technology project of Shandong Province (No. 2013WS0306).

Compliance with ethical standards

Conflict of interest

We declare that we have no conflicts of interest to this work, and have no financial and personal relationships with other people or organizations that can inappropriately influence our work.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Naili Zhang
    • 1
  • Lina Ma
    • 2
  • Xiaowei Liu
    • 1
  • Xiaorui Jiang
    • 3
  • Zhenhai Yu
    • 1
  • Dongmei Zhao
    • 1
  • Luping Zhang
    • 1
  • Chunlei Zhang
    • 1
  • Fei Huang
    • 1
    Email author
  1. 1.Department of Human Anatomy, School of Basic Medical ScienceBinzhou Medical UniversityYantaiChina
  2. 2.Department of Diagnostics, School of MedicineBinzhou Medical UniversityYantaiChina
  3. 3.Department of Hand and Foot SurgeryYuhuangding HospitalYantaiChina

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