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Cell and Tissue Banking

, Volume 19, Issue 4, pp 489–497 | Cite as

Effect of freezing and storage temperature on stability and antimicrobial activity of an antibiotic mixture used for decontamination of tissue allografts

  • Vicente Mirabet
  • Ana Melero
  • M. Dolores Ocete
  • Dimitra Bompou
  • Miriam Torrecillas
  • Juan J. Carreras
  • Isabel Valero
  • Ana I. Marqués
  • Rafael Medina
  • Luis R. Larrea
  • Cristina Arbona
  • Teresa M. Garrigues
  • Concepción Gimeno
Article

Abstract

One of the most important risks to be controlled in tissue banking is the infection associated with the clinical use of auto- and allografts. Thus, tissue disinfection protocols are used, in addition to processing in controlled environments. For this purpose, combinations of antibiotics are designed to ensure a broad spectrum of antimicrobial activity. This type of protocol is usually validated by testing its antimicrobial efficacy. In this work, we have studied the effect of several factors on the potential of an antibiotic mixture: container, freezing, storage at 4 °C, storage at − 30 °C and storage at − 80 °C. The molecular stability of the compounds has also been tested, additionally to their efficacy. Our findings show that storage conditions affect the molecular stability of Fungizone and Tobramycin (only in case of frozen storage for the last one). Nevertheless, the solution retains its antimicrobial activity for several weeks. The availability of stored aliquots of disinfectant solution and defining expiry dates for different storage conditions can help to schedule tissue bank tasks.

Keywords

Disinfection Allograft Tissue banking Storage Molecular stability Antimicrobial activity 

References

  1. Allen LV Jr (1997) Stability of vancomycin hydrochloride in medication cassette. Int J Pharm Compd 1(2):123–124Google Scholar
  2. Aponte-Tinao LA, Ayerza MA, Muscolo DL, Farfalli GL (2016) What are the risk factors and management options for infection after reconstruction with massive bone allografts? Clin Orthop Relat Res 474:669–673CrossRefGoogle Scholar
  3. Balamuralikrishna K, Syamasundar B (2010) Validated RP-HPLC method for the estimation of amphotericin B in bulk and pharmaceutical dosage form. http://citeweb.info/20101515503
  4. Brubaker S, Lotherington K, Zhao J, Hamilton B, Rockl G, Duong A, Garibaldi A, Simunovic N, Alsop D, Dao D, Bessemer R, Ayeni OR, Bioburden Steering Committee and Tissue Recovery Working Group (2016) Tissue recovery practices and bioburden: a systematic review. Cell Tissue Bank 17(4):561–571CrossRefGoogle Scholar
  5. Buzzi M, Guarino A, Gatto C, Manara S, Dainese L, Polvani G, Tóthová JD (2014) Residual antibiotics in decontaminated human cardiovascular tissues intended for transplantation and risk of falsely negative microbiological analyses. PLoS ONE 9(11):e112679CrossRefGoogle Scholar
  6. Clinical and Laboratory Standards Institute (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard–ninth edition. CLSI document M07-A9. Clinical and Laboratory Standards Institute, Wayne, PAGoogle Scholar
  7. Commission Directive 2006/86/EC of 24 October 2006 implementing Directive 2004/23/EC of the European Parliament and of the Council as regards traceability requirements, notification of serious adverse reactions and events and certain technical requirements for the coding, processing, preservation, storage and distribution of human tissues and cellsGoogle Scholar
  8. Coraça-Huber DC, Hausdorfer J, Fille M, Steidl M, Nogler M (2013) Effect of freezing on the release rate of gentamicin palmitate and gentamicin sulfate from bone tissue. Cell Tissue Bank 14(2):221–229CrossRefGoogle Scholar
  9. de By TM, Parker R, Delmo Walter EM, Hetzer R (2012) Cardiovascular tissue banking in Europe. HSR Proc Intensive Care Cardiovasc Anesth 4(4):251–260Google Scholar
  10. Eastlund T (2006) Bacterial infection transmitted by human tissue allograft transplantation. Cell Tissue Bank 7(3):147–166CrossRefGoogle Scholar
  11. European Directorate for the Quality of Medicines and HealthCare of the Council of Europe (2015) Guide to the quality and safety of tissues and cells for human application. Chapter 7: processing and storage. Strasbourg, FranceGoogle Scholar
  12. Fan YD, Van Hoeck B, Holovska V, Jashari R (2012) Evaluation of decontamination process of heart valve and artery tissues in European Homograft Bank (EHB): a retrospective study of 1,055 cases. Cell Tissue Bank 13(2):297–304CrossRefGoogle Scholar
  13. Germain M, Strong DM, Dowling G, Mohr J, Duong A, Garibaldi A, Simunovic N, Ayeni OR, Bioburden Steering Committee and Cardiac Tissue Working group (2016) Disinfection of human cardiac valve allografts in tissue banking: systematic review report. Cell Tissue Bank 17(4):593–601CrossRefGoogle Scholar
  14. Harmita H (2012) Optimation and validation of analytical method of co-trimoxazole in tablet and plasma in vitro by HPLC. J Bioanal Biomed 4:026–029.  https://doi.org/10.4172/1948-593X.1000059 CrossRefGoogle Scholar
  15. Heng WL, Albrecht H, Chiappini P, Lim YP, Manning L (2013) International heart valve bank survey: a review of processing practices and activity outcomes. J Transpl 2013:163150Google Scholar
  16. Hinsenkamp M, Muylle L, Eastlund T, Fehily D, Noël L, Strong DM (2012) Adverse reactions and events related to musculoskeletal allografts: reviewed by the World Health Organisation Project NOTIFY. Int Orthop 36(3):633–641CrossRefGoogle Scholar
  17. Holder IA, Robb E, Kagan R (1998) Antimicrobial mixtures used by tissue banks for harvested skin: comparative in vitro activity. Burns 24(7):604–608CrossRefGoogle Scholar
  18. Holder IA, Robb E, Kagan R (1999) Antimicrobial mixtures used to store harvested skin: antimicrobial activities tested at refrigerator (4 °C) temperatures. J Burn Care Rehabil 20(6):501–504CrossRefGoogle Scholar
  19. Ibrahim T, Stafford H, Esler CN, Power RA (2004) Cadaveric allograft microbiology. Int Orthop 28(5):315–318CrossRefGoogle Scholar
  20. Johnston C, Callum J, Mohr J, Duong A, Garibaldi A, Simunovic N, Ayeni OR, Bioburden Steering Committee and Skin Working group (2016) Disinfection of human skin allografts in tissue banking: a systematic review report. Cell Tissue Bank 17(4):585–592CrossRefGoogle Scholar
  21. Kainer MA, Linden JV, Whaley DN, Holmes HT, Jarvis WR, Jernigan DB, Archibald LK (2004) Clostridium infections associated with musculoskeletal-tissue allografts. N Engl J Med 350(25):2564–2571CrossRefGoogle Scholar
  22. Kuehnert MJ, Clark E, Lockhart SR, Soll DR, Chia J, Jarvis WR (1998) Candida albicans endocarditis associated with a contaminated aortic valve allograft: implications for regulation of allograft processing. Clin Infect Dis 27(4):688–691CrossRefGoogle Scholar
  23. Leeming JP, Lovering AM, Hunt CJ (2005) Residual antibiotics in allograft heart valve tissue samples following antibiotic disinfection. J Hosp Infect 60(3):231–234CrossRefGoogle Scholar
  24. Malinin TI, Buck BE, Temple HT, Martinez OV, Fox WP (2003) Incidence of clostridial contamination in donors’ musculoskeletal tissue. J Bone Joint Surg Br 85(7):1051–1054CrossRefGoogle Scholar
  25. Mankin HJ, Hornicek FJ, Raskin KA (2005) Infection in massive bone allografts. Clin Orthop Relat Res 432:210–216CrossRefGoogle Scholar
  26. Mirabet V, Alvarez M, Solves P, Ocete D, Gimeno C (2012) Use of liquid nitrogen during storage in a cell and tissue bank: contamination risk and effect on the detectability of potential viral contaminants. Cryobiology 64(2):121–123CrossRefGoogle Scholar
  27. Mohr J, Germain M, Winters M, Fraser S, Duong A, Garibaldi A, Simunovic N, Alsop D, Dao D, Bessemer R, Ayeni OR, Bioburden Steering Committee and Musculoskeletal Tissue Working group (2016) Disinfection of human musculoskeletal allografts in tissue banking: a systematic review. Cell Tissue Bank 17(4):573–584CrossRefGoogle Scholar
  28. Paolin A, Trojan D, Petit P, Coato P, Rigoli R (2017) Evaluation of allograft contamination and decontamination at the Treviso Tissue Bank Foundation: a retrospective study of 11,129 tissues. PLoS ONE 12(3):e0173154CrossRefGoogle Scholar
  29. Pitt TL, Tidey K, Roy A, Ancliff S, Lomas R, McDonald CP (2014) Activity of four antimicrobial cocktails for tissue allograft decontamination against bacteria and Candida spp. of known susceptibility at different temperatures. Cell Tissue Bank 15(1):119–125CrossRefGoogle Scholar
  30. Ruckmani K, Shaikh SZ, Khalil P, Muneera M (2011) A simple and rapid HPLC method for determining tobramycin in pharmaceutical formulations by direct UV detection. Pharm Methods 2(2):117CrossRefGoogle Scholar
  31. Schubert T, Bigaré E, Van Isacker T, Gigi J, Delloye C, Cornu O (2012) Analysis of predisposing factors for contamination of bone and tendon allografts. Cell Tissue Bank 13(3):421–429CrossRefGoogle Scholar
  32. Segur JM, Suso S, García S, Combalía A, Fariñas O, Llovera A (2000) The procurement team as a factor of bone allograft contamination. Cell Tissue Bank 1(2):117–119CrossRefGoogle Scholar
  33. Steffen V, Marsch G, Burgwitz K, Kuehn C, Teebken OE (2016) Resistance to infection of long-term cryopreserved human aortic valve allografts. J Thorac Cardiovasc Surg 151(5):1251–1259CrossRefGoogle Scholar
  34. Terzaghi C, Longo A, Legnani C, Bernasconi DP, Faré M (2015) Incidence of bacterial contamination and predisposing factors during bone and tendon allograft procurement. Cell Tissue Bank 16(1):151–157CrossRefGoogle Scholar
  35. Udekwu KI, Parrish N, Ankomah P, Baquero F, Levin BR (2009) Functional relationship between bacterial cell density and the efficacy of antibiotics. J Antimicrob Chemother 63(4):745–757CrossRefGoogle Scholar
  36. Witsø E, Persen L, Benum P, Bergh K (2005) Cortical allograft as a vehicle for antibiotic delivery. Acta Orthop 76(4):481–486CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Vicente Mirabet
    • 1
  • Ana Melero
    • 2
  • M. Dolores Ocete
    • 3
    • 5
  • Dimitra Bompou
    • 2
  • Miriam Torrecillas
    • 3
  • Juan J. Carreras
    • 2
  • Isabel Valero
    • 3
  • Ana I. Marqués
    • 4
  • Rafael Medina
    • 3
  • Luis R. Larrea
    • 1
  • Cristina Arbona
    • 1
  • Teresa M. Garrigues
    • 2
  • Concepción Gimeno
    • 3
    • 6
  1. 1.Cell and Tissue BankCentro de Transfusión de la Comunidad ValencianaValènciaSpain
  2. 2.Department of Pharmacy, Pharmaceutical Technology and Parasitology, Facultat de FarmàciaUniversitat de ValènciaValènciaSpain
  3. 3.Service de MicrobiologyConsorcio Hospital General Universitario de ValènciaValènciaSpain
  4. 4.Instituto de Investigación Sanitaria La FeValènciaSpain
  5. 5.Facultad de MedicinaUniversidad Católica de ValènciaValènciaSpain
  6. 6.Department of Microbiology, Facultat de Medicina i OdontologiaUniversitat de ValènciaValènciaSpain

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