Deep frozen amniotic membrane used as a scaffold and/or carrier for different cell types
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Amniotic membrane is a biological material widely used in plastic and reconstructive surgery and in ophthalmology. Due to its excellent biocompatibility and strength we tried to use it as a scaffold for the in vitro cultivation of different cell types, especially keratinocytes and limbal stem cells. It was possible to cultivate limbal stem cells and keratinocytes without using 3T3 mouse fibroblast feeder cells on deep frozen amniotic membranes. The amniotic membrane can also be used as a carrier for suspensions of different types of cells, allowing a substantial reduction of the cultivation time needed to prepare cell cultures for clinical application to burn patients. Our results show that the amniotic membrane seems not only to be an excellent carrier for human keratinocytes and corneal limbal stem cells, but also for other cell types, including dermal fibroblasts, adipose tissue-derived mesenchymal stem cells and chondrocytes.
KeywordsAmniotic membrane Keratinocytes Limbal stem cells Dermal fibroblasts Mesenchymal stem cells Chondrocytes
Central Tissue Bank
Limbal stem cells
Mesenchymal stem cells
Human dermal fibroblasts
- R-G method
Cultivation by Rheinwald and Green
This contribution is the result of the project implementation “Development of the Centre of Excellence for Exploitation of Informational Biomacromolecules for Improvement of Quality of Life” supported by the Research and Development Operational Programe funded by the ERDF (Contract No. ITMS 26240120027) and project “Irradiated Scaffolds for Tissue Engineering of Skin” supported by IAEA (International Atomic energy Agency) Contract Nr: 18282, CRP Code: E 31007.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflicts of interest.
Human and animal rights
This article does not contain any studies with human participants or animals performed by any of the authors.
- Baradaran-Raffi A, Aghayan HR, Arjmand B, Javadi MA (2007) Amniotic membrane transplantation. Iran J Ophtalmic Res 2(1):58–75Google Scholar
- Cameron PU, Pagnon JC, vanBaare J, Reece JC, Vardaxis NJ, Crowe SM (2000) Efficacy and kinetics of glycerol inactivation of HIV-1 in split skin grafts. J Med Virol 60:182–188. https://doi.org/10.1002/(SICI)1096-9071(200002)60:23.0.CO;2-Y CrossRefGoogle Scholar
- Davis J (1910) Skin transplantation with a review of 550 cases at the John Hopkins Hospital. Johns Hopkins Med J 15:307–315Google Scholar
- Dino BR, Eufemio G, DeVilla M, Reysio-Cruz M, Jurado RA (1965) The use of fetal membrane homografts in the local management of burns. J Philipp Med Assoc 41(12):890–898Google Scholar
- Ganatra MA (2003) Amniotic membrane in surgery. J Pak Med Assoc 53(1):29–32Google Scholar
- Hubka K, Dahlin RL, Meretoje VV, Kasper FK, Mikos AG (2014) Enhancing chondrogenic phenotype for cartilage tissue engineering: monocultire and coculture of articular chondrocytes and mesenchymal stem cells. Tissue Eng Part B 20(6):641–654. https://doi.org/10.1089/ten.TEB.2014.0034 CrossRefGoogle Scholar
- Koizumi NJ, Inatomi TJ, Sotozono CJ, Fullwood NJ, Quantock AJ, Kinoshita S (2000) Growth factor mRNA and protein in preserved human amniotic membrane. Curr Eye Res 20(3):173–177. https://doi.org/10.1076/0271-3683(200003)20:3;1-9;FT173 CrossRefGoogle Scholar
- Koller J, Panáková E (1997) Experience in the use of foetal membranes for the treatment of burns and other skin defects. In: Phillips GO, Strong DM, Versen RV, Nather A (eds) Advances in tissue banking, vol 2. World Scientific, Singapore, pp 353–359Google Scholar
- Mrázová H, Koler J, Kubisova K, Fujerikova G, Klincova E, Babal P (2016) Comparison of structural changes in skin and amnion tissue grafts for transplantation induced by gamma and electron beam irradiation for sterilisation. Cell Tissue Bank 17(2):255–260. https://doi.org/10.1007/s10561-015-9536-3 CrossRefGoogle Scholar
- Quershi IZ, Ambreen F, Khan WA (2010) Technique for Processing and preservation of human amniotic membrane for ocular surface reconstruction. World Acad Sci Eng Technol 69:763–766Google Scholar
- Rötth DA (1940) Plastic repair of conjunctival defects with fetal membranes. Arch Ophthalmol 23:522–525. https://doi.org/10.1001/archopht.1940.00860130586006 CrossRefGoogle Scholar
- Sabella N (1913) Use of the fetal membrane in skin grafting. Medicat Reconcil NY 83:478–480Google Scholar
- Shahdadfar A, Haug K, Pathak M, Drolsum L, Olstad OK, Johnsen EO, Petrovski G, Moe MC, Nicolaissen B (2012) Ex vivo expanded autologous limbal epithelial cells on amniotic membrane using a culture medium with human serum as single supplement. Exp Eye Res 97(1):1–9. https://doi.org/10.1016/j.exer.2012.01.013 CrossRefGoogle Scholar