Abstract
Tissue bioengineering development is a global concern and different materials are studied and created to be safe, effective and with low cost. Nile Tilapia skin had shown its biological potential as covers for the burn wound. This study evaluates the tilapia skin histological, collagen properties and tensiometric resistance, after treatment by different sterilization methods. Tilapia skin samples were submitted to two sterilization processes: (1) chemical, which consisted in two 2% chlorhexidin baths, followed by sequential baths in increasing glycerol concentrations; and (2) radiation, when glycerolized skin samples were submitted to gamma radiation at 25, 30 and 50 kGy. Microscopic analyzes were performed through Haematoxylin–eosin and Picrosirius Red under polarized light. For tensiometric analysis, traction tests were performed. Glycerol treated skin presented a discrete collagen fibers disorganization within the deep dermis, while irradiated skin did not show any additional change. Throughout the steps of chemical sterilization, there was a higher proportion of collagen with red/yellow birefringence (type I) in the skin samples up to the first bath in chlorhexidin, when compared to samples after the first two glycerol baths (P < 0.005). However, there was no difference in relation to total collagen between groups. In irradiated skin, there was a larger total collagen preservation when using until 30 kGy (P < 0.005). Tensiometric evaluation did not show significant differences in relation to maximum load in the groups studied. We concluded that chemical and radiation (25 and 30 kGy) are efficient methods to sterilize Nile Tilapia skin without altering its microscopic or tensiometric characteristics.
Similar content being viewed by others
References
Acar A, Uygur F, Diktaş H et al (2011) Comparison of silver-coated dressing (Acticoat®), chlorhexidine acetate 0.5% (Bactigrass®) and nystatin for topical antifungal effect in Candida albicans-contaminated, full-skin-thickness rat burn wounds. Burns 37(5):881–884. https://doi.org/10.1016/j.burns.2011.01.024
Alomar ZA, Gawri R, Roughley PJ, Haglund L, Burman M (2012) The effects of chlorhexidine graft decontamination on tendon graft. Am J Sports Med 40(7):1646–1653. https://doi.org/10.1177/0363546512443808
Baker TF, Ronholdt CJ, Bogdansky S (2005) Validating a low dose gamma irradiation process for sterilizing allografts using ISO 11137 Method 2B. Cell Tissue Bank 6(4):271–275. https://doi.org/10.1007/s10561-005-7364-6
Boateng J, Catanzano O (2015) Advanced therapeutic dressings for effective wound healing - a review. J Pharm Sci 104(11):3653–3680. https://doi.org/10.1002/jps.24610
Burton B, Gaspar A, Josey D, Tupy J, Grynpas MD, Willett TL (2014) Bone embrittlement and collagen modi fi cations due to high-dose gamma-irradiation sterilization. Bone 61:71–81. https://doi.org/10.1016/j.bone.2014.01.006
Chiu T, Burd A (2005) “Xenograft” dressing in the treatment of burns. Clin Dermatol 23(4):419–423. https://doi.org/10.1016/j.clindermatol.2004.07.027
Conrad BP, Rappé M, Horodyski M, Farmer KW, Indelicato PA (2013) The effect of sterilization on mechanical properties of soft tissue allografts. Cell Tissue Bank 14(3):359–366. https://doi.org/10.1007/s10561-012-9340-2
Delgado LM, Pandit A, Zeugolis DI (2014) Influence of sterilisation methods on collagen-based devices stability and properties. Expert Rev Med Devices 11(3):305–314. https://doi.org/10.1586/17434440.2014.900436
Endres S, Kratz M (2009) Gamma irradiation. An effective procedure for bone banks, but does it make sense from an osteobiological perspective? J Musculoskelet Neuronal Interact 9(1):25–31
Franco MLRS, Franco NP, Gasparino E, Dorado DM, Prado M, Vesco APD (2013) Comparação das peles de tilápia do Nilo, PACU e Tambaqui: histologia, composição e resistência. Arch Zootec 62(237):21–32
Guo Z-Q, Qiu L, Gao Y et al (2016) Use of porcine acellular dermal matrix following early dermabrasion reduces length of stay in extensive deep dermal burns. Burns. https://doi.org/10.1016/j.burns.2015.10.018
Inoue Y, Hasegawa M, Maekawa T et al (2016) The wound/burn guidelines–1: wounds in general. J Dermatol. https://doi.org/10.1111/1346-8138.13276
Kattz J (2010) The effects of various cleaning and sterilization processes on allograft bone incorporation. J Long Term Eff Med Implants. 20(4):271–276. http://www.ncbi.nlm.nih.gov/pubmed/21488820. Accessed 18 Feb 2017
Kesting MR, Wolff K-D, Hohlweg-Majert B, Steinstraesser L (2008) The role of allogenic amniotic membrane in burn treatment. J Burn Care Res 29(6):907–916. https://doi.org/10.1097/BCR.0b013e31818b9e40
Lineen E, Namias N (2008) Biologic dressing in burns. J Craniofac Surg 19(4):923–928. https://doi.org/10.1097/SCS.0b013e318175b5ab
Mann-Salinas EA, Joyner DD, Guymon CH et al (2015) Comparison of Decontamination Methods for Human Skin Grafts. J Burn Care Res 36(6):636–640. https://doi.org/10.1097/BCR.0000000000000188
Maral T, Borman H, Arslan H, Demirhan B (1999) Efectiveness of human amnion preserved long-term in glycerol as a temporary biological dressing. Burns 25(7):625–635
Mathangi Ramakrishnan K, Babu M, Mathivanan Jayaraman V, Shankar J (2013) Advantages of collagen based biological dressings in the management of superficial and superficial partial thickness burns in children. Ann Burns Fire Disasters 26(2):98–104
Nguyen H, Cassady AI, Bennett MB et al (2013) Reducing the radiation sterilization dose improves mechanical and biological quality while retaining sterility assurance levels of bone allografts. Bone 57(1):194–200. https://doi.org/10.1016/j.bone.2013.07.036
Norbury W, Herndon DN, Tanksley J, Jeschke MG, Finnerty CC (2016) Infection in Burns. Surg Infect (Larchmt) 17(2):250–255. https://doi.org/10.1089/sur.2013.134
Nunes Alves APN, Lima Verde MEQ, Ferreira Júnior AEC et al (2015) Avaliação microscópica, estudo histoquímico e análise de propriedades tensiométricas da pele de tilápia do Nilo. Rev Bras Queimaduras 14(3):203–210
Paggiaro AO, Mathor MB, de Carvalho VF (2010) Estabelecimento de protocolo de glicerolização de membranas amnióticas para uso como curativo biológico. Rev Bras Queimaduras 9(4):2–6
Ravishanker R, Bath AS, Roy R (2003) “Amnion Bank”–The use of long term glycerol preserved amniotic membranes in the management of superficial and superficial partial thickness burns. Burns 29(4):369–374. https://doi.org/10.1016/S0305-4179(02)00304-2
Shah NB, Wolkers WF et al (2009) Fourier transform infrared spectroscopy investigation of native tissue matrix modifications using a gamma irradiation process. Tissue Eng Part C Methods 15(1):33–40. https://doi.org/10.1089/ten.tec.2008.0158
Singh R, Singh D, Singh A (2016) Radiation sterelization of tissue allografts: a review. World J Radiol 8(4):355–370. https://doi.org/10.4329/wjr.v8.i4.355
Ülkür E, Öncül O, Karagöz H, Çeliköz B, Çavuslu S (2005) Comparison of silver-coated dressing (acticoat), chlorhexidine acetate 0.5% (bactigrass), and silver sulfadiazine 1% (silverdin) for topical antibacterial effect in pseudomonas aeruginosa-contaminated, full-skin thickness burn wounds in rats. J Burn Care Rehabil 26(5):430–433. https://doi.org/10.1097/01.bcr.0000176879.27535.09
von Versen-Hoeynck F, Per A, Becker J (2008) Sterilization and preservation influence the biophysical properties of human amnion grafts. Biologicals 36(4):248–255. https://doi.org/10.1016/j.biologicals.2008.02.001
Wasiak J, Cleland H, Campbell F (2008) Dressings for treating superficial and partial thickness burns. Cochrane Database Syst Rev 8(4):CD002106. https://doi.org/10.1002/14651858.CD002106.pub3
Zidan SM, Eleowa SA (2014) Banking and use of glycerol preserved full-thickness skin allograft harvested from body contouring procedures. Burns 40(4):641–647. https://doi.org/10.1016/j.burns.2013.08.039
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Alves, A.P.N.N., Lima Júnior, E.M., Piccolo, N.S. et al. Study of tensiometric properties, microbiological and collagen content in nile tilapia skin submitted to different sterilization methods. Cell Tissue Bank 19, 373–382 (2018). https://doi.org/10.1007/s10561-017-9681-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10561-017-9681-y