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Detection of hepatitis B virus in bone allografts from donors with occult hepatitis B infection

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Abstract

The implementation of nucleic acid testing in donor screening has improved the safety of tissue allografts. Although infectious disease transmission can be considered a rare event, the detection of occult hepatitis B infection remains challenging. The studies concerning this risk are mainly based on testing blood specimens. This work shows the correlation between results of samples obtained from donor blood and the corresponding tissue washing solution. Hepatitis B virus deoxyribonucleic acid was detected both in bone allografts from donors with serological profiles associated to active hepatitis B infection and occult hepatitis B infection. These results suggest that hepatitis B virus seems to concentrate in bone marrow even when a low viral load is present in peripheral blood. Even detection at molecular level is not enough to avoid the risk of hepatitis B virus transmission and a multiparametrical evaluation is required in tissue donor screening. The role of clinicians in recognition and reporting of allograft-associated infections is a major concern for the acquisition of experience to be applied in risk control of disease transmission.

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Authors and Affiliations

  1. Banco de Tejidos y Células de la Comunidad Valenciana, Avenida del Cid, 65-A, 46014, Valencia, Spain

    Vicente Mirabet, Manuel Álvarez, Mar Luis-Hidalgo, Nieves Puig, Luis Larrea & Cristina Arbona

  2. Hospital Universitari i Politècnic La Fe, Valencia, Spain

    Juan Galán

Authors
  1. Vicente Mirabet
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  2. Manuel Álvarez
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  3. Mar Luis-Hidalgo
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  4. Juan Galán
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  5. Nieves Puig
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  6. Luis Larrea
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  7. Cristina Arbona
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Correspondence to Vicente Mirabet.

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Mirabet, V., Álvarez, M., Luis-Hidalgo, M. et al. Detection of hepatitis B virus in bone allografts from donors with occult hepatitis B infection. Cell Tissue Bank 18, 335–341 (2017). https://doi.org/10.1007/s10561-017-9644-3

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  • DOI: https://doi.org/10.1007/s10561-017-9644-3

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