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Cell and Tissue Banking

, Volume 16, Issue 3, pp 335–342 | Cite as

Neurogenic differentiation from adipose-derived stem cells and application for autologous transplantation in spinal cord injury

  • Yong Zhao
  • Hui Jiang
  • Xin-wei Liu
  • Jian-Ting Chen
  • Liang-Bi Xiang
  • Da-Peng Zhou
Original Paper

Abstract

Mesenchymal stem cells derived from adipose tissue have the capacity to differentiate into endodermal, mesoderm and ectodermal cell lineages in vitro, which are an ideal engraft in tissue-engineered repair. In this study, mouse adipose-derived stem cells (ADSCs) were isolated from subcutaneous fat. The markers of ADSCs, CD13, CD29, CD44, CD71, CD73, CD90, CD105, CD166, Nestin, GFAP and MAP-2 were detected by immunofluorescence assays. The ADSCs were cultured in cocktail factors (including ATRA, GGF-2, bFGF, PDGF and forskolin) for neurogenic differentiation. The neurogenic cells markers, Nestin, GFAP and MAP-2 were analyzed using immunofluorescence and real-time PCR after dramatic changes in morphology. Neurogenic cells from ADSCs were autologous transplanted into the mouse of spinal cord injury for observation neurogenic cells colonization in spinal cord. The result demonstrated that the mouse ADSCs were positive for the CD13, CD29, CD44, CD71, CD73, CD90, CD105 and CD166 but negative for neurogenic cell markers, MAP-2, GFAP and Nestin. After neurogenic differentiation, the neurogenic cells were positive for neurogenic cell special markers, gene expression level showed a time-lapse increase, and the cells were successful colonized into spinal cord. In conclusion, our research shows that a population of neuronal cells can be specifically generated from ADSCs and that induced cells may allow for participation in tissue-repair.

Keywords

Adipose-derived stem cells Neurogenic differentiation Autologous transplantation 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Yong Zhao
    • 1
    • 2
  • Hui Jiang
    • 2
  • Xin-wei Liu
    • 1
  • Jian-Ting Chen
    • 2
  • Liang-Bi Xiang
    • 1
  • Da-Peng Zhou
    • 1
  1. 1.Department of OrthopedicsNanfang Hospital of Southern Medical UniversityGuangzhouChina
  2. 2.Department of Orthopedics, General Hospital of Shenyang Military Area Command of Chinese PLARescue Center of Severe Wound and Trauma of Chinese PLAShenyangChina

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