Cell and Tissue Banking

, Volume 15, Issue 4, pp 573–578 | Cite as

Comparison of organ cultured precut corneas versus surgeon-cut corneas for Descemet’s stripping automated endothelial keratoplasty

  • Suganiah Ragunathan
  • Anders Ivarsen
  • Kim Nielsen
  • Jesper Hjortdal
Original Paper


To compare precut and surgeon-cut organ cultured donor corneas for DSAEK. A total of 119 consecutive eyes treated with DSAEK were retrospectically identified. 65 grafts were cut by the surgeon (Moria, ALTK System) prior to DSAEK and 54 grafts were precut by laboratory technicians from the Danish Eye Bank (Horizon single-use system). 1 year after surgery, tomographic images were obtained with the Pentacam HR. Endothelial cell density (ECD) and best-corrected visual acuity (BCVA) was determined. Graft thickness and graft asymmetry was evaluated in the centre and 1 mm from the edge of the graft in 6 semi-meridians. 1 year after surgery, the ECD loss was similar in the two groups, averaging 25.9 ± 14 % in surgeon-cut, and 22.9 ± 17 % in precut group (p = 0.33). Mean central graft thickness was 172 ± 6 μm in surgeon-cut grafts and 182 ± 6 μm in precut grafts (p = 0.30). BCVA was similar in surgeon-cut and precut corneas; being 0.25 ± 0.02 logMAR and 0.24 ± 0.02 logMAR, respectively (p = 0.59). The graft asymmetry index was 1.48 ± 0.02 for surgeon-cut and 1.44 ± 0.02 for precut grafts. There were no significant differences in complications rate in both groups. No correlations between BCVA and central graft thickness or graft asymmetry index in both groups were observed. Organ cultured precut donor corneas are comparable with surgeon-cut grafts with respect to ECD, graft thickness and asymmetry, and postoperative complication rate.


Descemet stripping endothelial keratoplasty Corneal transplantation Eye banks Tissue donors Organ culture 



The Danish Association for Prevention of Blindness and Bagenkop Nielsens Foundation.

Conflict of interest

The authors have no financial disclosures.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Suganiah Ragunathan
    • 1
  • Anders Ivarsen
    • 1
  • Kim Nielsen
    • 1
  • Jesper Hjortdal
    • 1
  1. 1.Department of OphthalmologyAarhus University HospitalÅrhusDenmark

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