Cell and Tissue Banking

, Volume 15, Issue 2, pp 177–192 | Cite as

Amniotic membrane properties and current practice of amniotic membrane use in ophthalmology in Slovenia

  • Tina Cirman
  • Matej Beltram
  • Petra Schollmayer
  • Primož Rožman
  • Mateja Erdani Kreft
Original Paper


Amniotic membrane (AM) is the innermost, multilayered part of the placenta. When harvested, processed and stored properly, its properties, stemming from AM biological composition, make it a useful tissue for ophthalmic surgery. AM was shown to have several beneficial effects: it promotes epithelization, has antimicrobial effects, decreases inflammation, fibrosis and neovascularization. Many case reports and case series as well as practical experience (e.g. reconstruction of conjunctival and corneal defects, treatment of corneal ulcers) demonstrated the beneficial effect of AM for different ophthalmological indications. The combination of the above mentioned beneficial effects and reasonable mechanical properties are also the reason why AM is used as a substrate for ex vivo expansion of epithelial progenitor cells. Recently, amnion-derived cells, which also have stem cell characteristics, have been proposed as potential contributors to cell-based treatment of ocular surface disease. However, the use of AM remains one of the least standardized methods in ophthalmic surgery. In this review, the various properties of AM and its current clinical use in ophthalmology in Slovenia are discussed.


Amniotic membrane Ophthalmology Regenerative medicine Cornea Conjunctiva Amniotic stem cells 



The authors would like to express their appreciation to all who contributed to the development of this field of research and therapy in Slovenia. We thank Sabina Železnik for the technical assistance. The work was supported by the Slovenian Research Agency (Grant No P3-0108).


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Tina Cirman
    • 1
  • Matej Beltram
    • 2
  • Petra Schollmayer
    • 2
  • Primož Rožman
    • 1
    • 3
  • Mateja Erdani Kreft
    • 4
  1. 1.Blood Transfusion Centre of SloveniaLjubljanaSlovenia
  2. 2.Eye HospitalUniversity Medical Centre, LjubljanaLjubljanaSlovenia
  3. 3.University of LjubljanaFaculty of MedicineLjubljanaSlovenia
  4. 4.University of LjubljanaFaculty of Medicine, Institute of Cell BiologyLjubljanaSlovenia

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