Gentamicin palmitate as a new antibiotic formulation for mixing with bone tissue and local release
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During surgery with bone grafting, the impaction of bone tissue creates an avascular area where local circulation is disrupted. If infections arise, they may prevent systemically administered antibiotics from reaching the infected bone. In this study we evaluated gentamicin palmitate (GP) mixed with gentamicin sulfate (GS) as a coating for bone chips (BCh). The efficacy of the coated BCh was measured by gentamicin base release tests using B. subtilis, S. epidermidis and S. aureus. Gentamicin base release was evaluated in phosphate-buffered saline for up to 7 days using B. subtilis bioassay. Antimicrobial efficacy was tested with S. aureus and S. epidermidis. A significant difference on the release of gentamicin base between GS and GS + GP was observed. S. epidermidis are significantly more susceptible to GS + GP and GS than S. aureus. BCh can act as gentamicin carriers and showed efficacy against S. aureus and S. epidermidis.
KeywordsBone allografts Anti-infective coating Gentamicin palmitate Bacillus subtilis Staphylococcus aureus Staphylococcus epidermidis
We thank Dennis Huber, Experimental Orthopedics, Medical University Innsbruck and Sebastian Vogt from Heraeus Medical GmbH for their comments and improvement of this manuscript This study was funded by Heraeus Medical GmbH, Wehrheim, Germany. Dr. Débora C. Coraça-Huber, Dr. David Putzer and Dr. Michael Nogler are paid employees of Medical University Innsbruck, Experimental Orthopedics. Dr. Manfred Fille and Dr. Hausdorfer are paid employees of Medical University Innsbruck, Division of Hygiene and Medical Microbiology. Dr. Klaus-Dieter Kühn is paid employee by Heraeus Medical GmbH.
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