Cell and Tissue Banking

, Volume 15, Issue 1, pp 51–57 | Cite as

Repairing cartilage defects with bone marrow mesenchymal stem cells induced by CDMP and TGF-β1

  • Gang Wu
  • Ying Cui
  • Linxiang Ma
  • Xinyu Pan
  • Xuefeng Wang
  • Ben Zhang
Original Paper
First Online:
Received:
Accepted:

Abstract

The aim of this study was to explore the ability for chondrogenic differentiation of bone marrow mesenchymal stems cells (BMSCs) induced by either cartilage-derived morphogenetic protein 1 (CDMP-1) alone or in the presence of transforming growth factor-β1 (TGF-β1) in vivo and in vitro. BMSCs and poly-lactic acid/glycolic acid copolymer (PLGA) scaffold were analyzed for chondrogenic capacity induced by CDMP-1 and TGF-β1 in vivo and in vitro. Chondrogenic differentiation of BMSCs into chondrocytes using a high density pellet culture system was tested, whether they could be maintained in 3-D PLGA scaffold instead of pellet culture remains to be explored. Under the culture of high-density cell suspension and PLGA frame, BMSCs were observed the ability to repair cartilage defects by either CDMP-1 alone or in the presence of TGF-β1 in vitro. Then the cell-scaffold complex was implanted into animals for 4 and 8 weeks for in vivo test. The content of collagen type II and proteoglycan appeared to increase over time in the constructs of the induced groups (CDMP in the presence of TGF-β1), CDMP group and TGF group. However, the construct of the control group did not express them during the whole culture time. At 4 and 8 weeks, the collagen type II expression of the induced group was higher than the sum of TGF group and CDMP group by SSPS17.0 analysis. BMSCs and PLGA complex induced by CDMP-1 and TGF- β1 can repair cartilage defects more effectively than that induced by CDMP-1 or TGF-β1 only.

Keywords

Bone marrow mesenchymal stem cells Cartilage-derived morphogenetic protein-1 Transforming growth factor-β1 Poly-lactic acid/glycolic acid copolymer Chondrogenic differentiation 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This study was supported by the Grant of Education Department of Liaoning Province (No: LT2010063, 2010T110) and Science and Technology Department of Liaoning Province (No: 20062198).

References

  1. An C, Cheng Y, Yuan Q, Li J (2010) IGF-1 and BMP-2 induces differentiation of adipose-derived mesenchymal stem cells into chondrocytes-like cells. Ann Biomed Eng 38:1647–1654PubMedCrossRefGoogle Scholar
  2. Bai X, Xia Z, Pan Y, Hu J, Pohl J, Wen J, Li L (2004) Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes. Biochem Biophys Res Commun 325:453–460PubMedCrossRefGoogle Scholar
  3. Bobaca K, Ullrich R, Amoyo L, Erlacher L, Smolen JS, Graninger WB (2006) Stimulatory effects of distinct members of the bone morphogenetic protein family on ligament fibroblasts. Ann Rheum Dis 65:169–177CrossRefGoogle Scholar
  4. Cui Y, Wang H, Yu M, Xu T, Li X, Li L (2006) Differentiation plasticity of human fetal articular chondrcytes. Otolaryngol Head Neck Surg 135:61–67PubMedCrossRefGoogle Scholar
  5. Cui M, Wan Y, Anderson DG, Shen FH, Leo BM, Laurencin CT, Balian G, Li X (2007) Mouse growth and differentiation factor-5 protein and DNA therapy potentiates intervertebral disc cell aggregation and chondrogenic gene expression. Spine J 8:287–295PubMedCrossRefGoogle Scholar
  6. Gantenbein-Ritter B, Benneker LM, Alini M, Grad S (2011) Differential response of human bone marrow stromal cells to either TGF-β (1) or rhGDF-5. Eur Spine J 20:962–971PubMedCentralPubMedCrossRefGoogle Scholar
  7. Gruber R, Mayer C, Schulz W (2000) Stimulatory effects of cartilage-derived morphogenetic proteins 1 and 2 on osteogenic differentiation of bone marrow stromal cells. Cytokine 12:1630–1638PubMedCrossRefGoogle Scholar
  8. Guo CA, Liu XG, Huo JX, Jiang C, Wen XJ, Chen ZR (2007) Novel gene-modified- tissue engineering of cartilage using stable transforming growth factor-β1 transfected mesenchymal stem cells grown on chitosan scaffolds. J Biosci Bioeng 103:547–556PubMedCrossRefGoogle Scholar
  9. Liu FL, Lin LH, Sytwu HK, Chang DM (2010) GDF-5 is suppressed by IL-1β and enhances TGF-β3-mediated chondrogenic differentiation in human rheumatoid fibroblast-like synoviocytes. Exp Mol Pathol 88:163–170PubMedCrossRefGoogle Scholar
  10. Luyten FP (1997) Cartilage-derived morphogenetic protein-1. Int J Biochem Cell Biol 29:1241–1244PubMedCrossRefGoogle Scholar
  11. Mijkovic ND, Cooper GM, Marra KG (2010) Chondrogenesis, bone morphogenetic protein-4 and mesenchymal stem cells. Osteoarthr Cartil 16:1121–1130CrossRefGoogle Scholar
  12. Mori M, Motohashi M, Nishikawa T, Masuno K, Tanaka A, Ohta T, Sumitomo S, Shikimori M (2010) Biological implications of growth factors in bone remodeling following fracture, surgical resection and bonegrafting. Part 1: transforming growth factors, bone morphogenetic proteins and related factors. Asian J Oral Maxillofac Surg 22:117–125CrossRefGoogle Scholar
  13. Pabbruwe MB, Kafienah W, Tarlton JF, Mistry S, Fox DJ, Hollander AP (2010) Repair of meniscal cartilage white zone tears using a stem cell/collagen-scaffold implant. Biomaterials 31:2583–2591PubMedCrossRefGoogle Scholar
  14. Park KH, Na K (2008) Effect of growth factors on chondrogenic differentiation of rabbit mesenchymal cells embedded in injectable hydrogels. J Biosci Bioeng 106:74–79PubMedCrossRefGoogle Scholar
  15. Pittenger MF, Mackay AM, Beck SC et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147PubMedCrossRefGoogle Scholar
  16. Rouault K, Scotet V, Autret S, Gaucher F, Dubrana F, Tanquy D, Yaacoub C, Fenoll B, Ferec C (2010) Evidence of association between GDF5 polymorphisms and congenital dislocation of the hip in a Caucasian population. Osteoarthr Cartil 18:1144–1149PubMedCrossRefGoogle Scholar
  17. Stoyanov JV, Gantenbein-Ritter B, Bertolo A, Aebli N, Baur M, Alini M, Grad S (2011) Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cells. Eur Cell Mater 21:533–547PubMedGoogle Scholar
  18. Uebersax L, Merkle HP, Meinel L (2008) Insulin-like growth factors I releasing silk fibroin scaffolds induce chondrogenic differentiation of human mesenchymal stem cells. J Control Release 127:12–21PubMedCrossRefGoogle Scholar
  19. Yin S, Cen L, Wang C, Zhao G, Sun J, Liu W, Cao Y, Cui L (2010) Chondrogenic transdifferentiation of human dermal fibroblasts stimulated with cartilage-derived morphogenetic protein 1. Tissue Eng Part A 16:1633–1643PubMedCrossRefGoogle Scholar
  20. Zeng Q, Li X, Beck G, Balian G, Shen FH (2007) Growth and differentiation factor-5 (GDF-5) stimulates osteogenic differentiation and increases vascular endothelial growth factor (VEGF) levels in fat-derived stromal cells in vitro. Bone 40:374–381PubMedCrossRefGoogle Scholar
  21. Zhao G, Yin S, Liu G, Cen L, Sun J, Zhou H, Liu W, Cui L, Cao Y (2009) In vitro engineering of fibrocartilage using CDMP-1 induced dermal fibroblasts and polyglycolide. Biomaterials 30:3241–3250PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Gang Wu
    • 2
  • Ying Cui
    • 1
  • Linxiang Ma
    • 3
  • Xinyu Pan
    • 1
  • Xuefeng Wang
    • 1
  • Ben Zhang
    • 1
  1. 1.Department of OtolaryngologyThe First Affiliated Hospital of Liaoning Medical CollegeJinzhou CityChina
  2. 2.Department of SurgeryThe First Affiliated Hospital of Liaoning Medical CollegeJinzhou CityChina
  3. 3.Department of OtolaryngologyThe Affiliated Hospital of Jining Medical CollegeJining CityChina

Personalised recommendations