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Cell and Tissue Banking

, Volume 14, Issue 1, pp 77–84 | Cite as

Use of irradiated human amnion as a matrix for limbal stem cell culture

  • Carlos Landa-Solís
  • Leticia Vázquez-Maya
  • María Esther Martínez-Pardo
  • Ana M. Brena-Molina
  • Erika Ruvalcaba
  • Ricardo Gómez
  • Clemente Ibarra
  • Cristina Velasquillo
Original Paper

Abstract

Several ocular diseases affect the corneal surface; the development of effective technologies for the treatment of corneal lesions has brought about an improvement in the quality of life of affected patients. The aim of this study is to culture and characterize limbal stem cells cultured on gamma (60Co) radiosterilized human amnion (RHA). Limbal stem cells were isolated from ten preserved samples of corneal transplant. The cells were cultured since primary culture until expanded cells on RHA and stained with monoclonal antibodies to establish their immunophenotype, after which cytokeratin 12 and Vimentin were positive by immunohistochemistry. The immunophenotype remained constant since primary culture until expanded cells in RHA. The RHA and cells construct were structurally integrated. Immunohistochemistry was cytokeratin 12, Vimentin positive, and cytokeratin 19 negative. In vitro limbal cells maintain a constant epithelial transition immunophenotype in culture up to primary culture until expanded cells on RHA.

Keywords

Gamma radiation Cornea Radiosterilized human amnion Limbal stem cells 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Carlos Landa-Solís
    • 1
  • Leticia Vázquez-Maya
    • 2
  • María Esther Martínez-Pardo
    • 3
  • Ana M. Brena-Molina
    • 1
  • Erika Ruvalcaba
    • 1
  • Ricardo Gómez
    • 1
  • Clemente Ibarra
    • 1
  • Cristina Velasquillo
    • 1
  1. 1.Instituto Nacional de RehabilitacionMexicoMexico
  2. 2.Hospital General de MéxicoMexicoMexico
  3. 3.Instituto Nacional de Investigaciones NuclearesMexicoMexico

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