Cell and Tissue Banking

, Volume 12, Issue 3, pp 171–184 | Cite as

The comparative analysis of homologous fresh frozen bone and autogenous bone graft, associated or not with autogenous bone marrow, in rabbit calvaria: a clinical and histomorphometric study

  • André Antonio Pelegrine
  • Carlos Eduardo Sorgi da Costa
  • Wilson Roberto Sendyk
  • Alfredo Gromatzky
Original Paper

Abstract

The aim of this study was to evaluate the potential of fresh frozen homologous and autogenous grafts, associated or not with autogenous bone marrow, to form bone. Sixty titanium cylinders were used, and were fixed to the skulls of 30 rabbits. These cylinders were filled with (A) autogenous bone (AM) autogenous bone associated with the bone marrow (H) fresh frozen homologous bone (HM) fresh frozen homologous bone associated with the bone marrow (M) pure autogenous bone marrow and (C) blood clot. The animals were sacrificed after 02 and 03 months. After clinical evaluation, the samples were stained with hematoxylin, eosin and Mallory Trichrome dyes for optical microscopy analysis and histomorphometric analysis. Experimental groups that received mineralized materials (A, AM, H, HM) showed the best bone formation results, presenting no statistical difference between them (P > 0.05). Groups that did not receive mineralized materials (M and C) showed the worst results (P < 0.05), but the M group showed better results than the C group. Most of the autogenous and homologous bone particles were resorbed and there was a larger amount of residual particles in the homologous graft (H, HM) when compared with the autogenous graft (A, AM; P < 0.05). These findings suggest that fresh frozen homologous grafts produced similar amounts of new bone when compared with the autogenous grafts. However, the amount of residual bone particles was larger in the homogenous groups, which may indicate a slower remodeling process. The homologous fresh frozen bone seems to be a good osteoconductive material. The use of only autogenous bone marrow showed better results when compared to the bood clot. However, this research indicates that association with mineralized materials is required.

Keywords

Bone transplantation Bone marrow Homologous transplantation 

Notes

Acknowledgments

The authors acknowledge, with thanks, the assistance of Luiz Antonio Mazzuchelli Cosmo, Luís Guilherme Scavone de Macedo, Rafael Manfro and Renato Limberte, MSs. They also thank Dr Jacks Jorge for allowing the use of his lab and helping with the histology.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • André Antonio Pelegrine
    • 1
    • 4
  • Carlos Eduardo Sorgi da Costa
    • 2
  • Wilson Roberto Sendyk
    • 3
  • Alfredo Gromatzky
    • 3
  1. 1.São Leopoldo Mandic Dental SchoolCampinasBrazil
  2. 2.School of Medicine, UNIFESPSão PauloBrazil
  3. 3.Santo Amaro UniversitySão PauloBrazil
  4. 4.CampinasBrazil

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