Musculoskeletal allografts are a valuable alternative to autograft tissue in orthopaedic surgeries. However, the effects of the allografts’ storage history on the collagen and subsequent allograft scaffold properties are unknown. In this study, we hypothesized that freezing and refrigeration of allografts for 1 week would alter the biologic performance and mechanical properties of the allograft collagen. Allograft collagen was characterized by SDS–PAGE migration pattern, amino acid profile and measured denaturation. Scaffolds made from allograft collagen were evaluated for fibroblast proliferation, platelet activation and scaffold retraction. Collagen gelation kinetics (elastic and inelastic moduli and the viscous-elastic transition point) were also evaluated. Fibroblast proliferation, platelet activation and scaffold retraction results showed only minor, though statistically significant, differences between the storage groups. In addition, there were no significant differences in rheological properties or collagen biochemistry. In conclusion, this study suggests that freezing or refrigeration for 1 week does not appear to have any detrimental effect on the mechanical properties and biologic performance of the collagen within allografts.
Allograft Storage Collagen Scaffold
This work was supported by a grant from the Musculoskeletal Transplant Foundation. The authors thank Marie Torres (amino acid analysis), Yin Yin Lin and Zachary Waldon (SDS–PAGE), and the help of Dr. David Zurakowski with the statistical analyzes.
Bashey RI, Bashey HM et al (1978) Characterization of pepsin-solubilized bovine heart-valve collagen. Biochem J 173(3):885–894PubMedGoogle Scholar
Batge B, Winter C et al (1997) Glycosylation of human bone collagen I in relation to lysylhydroxylation and fibril diameter. J Biochem 122(1):109–115PubMedGoogle Scholar
Brighton CT, Shadle CA et al (1979) Articular cartilage preservation and storage. I. Application of tissue culture techniques to the storage of viable articular cartilage. Arthritis Rheum 22(10):1093–1101. doi:10.1002/art.1780221008PubMedCrossRefGoogle Scholar
Chandrakasan G, Torchia DA et al (1976) Preparation of intact monomeric collagen from rat tail tendon and skin and the structure of the nonhelical ends in solution. J Biol Chem 251(19):6062–6067PubMedGoogle Scholar
Li YY, Feng YQ et al (2000) Myocardial extracellular matrix remodeling in transgenic mice overexpressing tumor necrosis factor alpha can be modulated by anti-tumor necrosis factor alpha therapy. Proc Natl Acad Sci USA 97(23):12746–12751. doi:10.1073/pnas.97.23.12746PubMedCrossRefGoogle Scholar
Vassbotn FS, Havnen OK et al (1994) Negative feedback regulation of human platelets via autocrine activation of the platelet-derived growth factor alpha-receptor. J Biol Chem 269(19):13874–13879PubMedGoogle Scholar
Viidik A, Lewin T (1966) Changes in tensile strength characteristics and histology of rabbit ligaments induced by different modes of postmortal storage. Acta Orthop Scand 37(2):141–155PubMedCrossRefGoogle Scholar