Functional properties of fresh and cryopreserved carotid and femoral arteries, and of venous and synthetic grafts: comparison with arteries from normotensive and hypertensive patients
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The ideal arterial graft must share identical functional properties with the host artery. Surgical reconstruction of the common carotid artery (CA) is performed in several clinical situations, using expanded polytetrafluoroethylene prosthesis (ePTFE) or saphenous vein (SV) grafts. At date there is interest in obtaining an arterial graft that improves the results of that nowadays available. The use of a fresh or cryopreserved/defrosted artery appears as an interesting alternative. However, if the fresh and cryopreserved/defrosted arteries allow an adequate viscoelastic and functional matching with the host arteries needs to be established. The aims were to compare the viscoelastic and functional performance of: (1) conduits used in CA reconstruction (SV and ePTFE) with those of the fresh and cryopreserved/defrosted CA and femoral arteries (FA), and (2) normotensive and hypertensive patients’ arteries with those of the arterial substitutes in vitro analyzed. Pressure, diameter and wall thickness of the CA were recorded in 15 normotensive and 15 hypertensive patients (in vivo studies), and in SV, fresh and cryopreserved/defrosted CA and FA (obtained from 15 donors), and ePTFE segments (in vitro studies). From stress–strain relationship we calculated elastic and viscous modulus, and the characteristic impedance. The local buffer and conduit functions were quantified as the viscous/elastic quotient and the inverse of the characteristic impedance. Fresh and cryopreserved/defrosted CA and FA were more alike, both in viscoelastic and functional levels, respect to normotensive and hypertensive patients’ arteries, than the ePTFE and SV grafts. CA and FA cryografts could be considered an important alternative for carotid reconstruction.
KeywordsArterial wall Carotid bypass Carotid reconstruction Cryopreservation ePTFE Femoral artery Functional matching Saphenous vein Stress–strain Viscoelasticity
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- Armentano R.L., Barra J.G., Levenson J., Simon A. and Pichel RH. (1995a). Arterial wall mechanics in conscious dogs: assessment of viscous, inertial, and elastic moduli to characterize aortic wall behaviour. Circ. Res. 76, 468–478Google Scholar
- Armentano R., Megnien J.L., Simon A., Bellenfant F., Barra J. and Levenson J. (1995b). Effects of hypertension on viscoelasticity of carotid and femoral arteries in humans. Hypertension 26: 48–54Google Scholar
- Bia Santana D., Barra J.G., Grignola J.C., Gines F.F. and Armentano R.L. (2005a). Pulmonary artery smooth muscle activation attenuates arterial dysfunction during acute pulmonary hypertension. J. Appl. Physiol. 98: 605–613Google Scholar
- Bia D., Armentano R.L., Zócalo Y., Barmak W., Migliaro E. and Cabrera Fischer E.I. (2005b). In vitro model to study arterial wall dynamics through pressure-diameter relationship analysis. Latin Am. Appl. Res. 35: 217–224Google Scholar
- Bia D., Pessana F., Armentano R., Pérez H., Graf S., Zócalo Y., Saldías M., Pérez N., Alvarez O., Silva W., Machin D., Sueta P., Ferrin S., Acosta M. and Alvarez I. 2006. Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis. Cell Tissue Bank. (In press)Google Scholar
- Graf S., Gariepy J., Massoneau M., Armentano R.L., Masour S., Barra J.G., Simon A. and Levenson J. (1999). Experimental and clinical validation of arterial diameter waveform and intimal media thickness obtained from B-mode ultrasound image processing. Ultrasound Med. Biol. 25(9): 1353–1363PubMedCrossRefGoogle Scholar
- Mavrilas D. and Tsapikouni T. (2002). Dynamic mechanical properties of arterial and venous grafts used in coronary bypass surgery. J. Mech. Med. Biol. 2(3–4): 1–9Google Scholar
- Nichols W.W., O’Rourke M.F. (1998). Properties of the arterial wall: practice. In: Nichols WW, O’Rourke MF (eds) Mc Donald’s Blood Flow in Arteries Theoretical, Experimental and Clinical Principles. Arnold, London, pp. 73–97Google Scholar