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Cell and Tissue Banking

, Volume 7, Issue 1, pp 47–53 | Cite as

Clinical Results of an Autologous Engineered Skin

  • Sara Llames
  • Eva García
  • Verónica García
  • Marcela del Río
  • Fernando Larcher
  • Jose Luis Jorcano
  • Eva López
  • Purificación Holguín
  • Francisca Miralles
  • Jesús Otero
  • Alvaro MeanaEmail author
Article

Abstract

Introduction: An artificial complete skin (dermis and epidermis) model has been developed in the Tissue engineering unit of the Centro Comunitario de Sangre y Tejidos del Principado de Asturias (CCST) and CIEMAT. This engineered skin has been employed for the treatment of severe epithelial injuries. In this paper, the clinical results obtained with this engineered skin during the last 18 months were evaluated. Patients, material and methods: (a) Culture: Cells (fibroblasts and keratinocytes) were obtained from biopsies by a double enzymatic digestion. After an expansion period, fibroblasts were seeded in an artificial dermis based on human plasma. Keratinocytes were seeded over this dermal surface. (b) Patients: 20 skin biopsies were processed (13 burned patients, 5 giant nevus, 1 GVHD, 1 neurofibromatosis), which came from different hospitals across the country. About 97,525 cm2 of engineered skin were cultured. Results: The engineered skin took in all patients. The take percentage depended on previous pathology (burned patients 10–90%; non-critical patients 70–90%). The epithelization obtained was permanent in all cases. During the follow-up period, epithelial loss, blistering injuries or skin retractions were not observed. The aesthetic and functional results were acceptable. Conclusions: This artificial skin has demonstrated to be useful for the definitive treatment of patients with severe skin injuries. This work shows that it is possible to produce this prototype in an hospitalarian laboratory and distribute it to different hospitals across the country.

Keywords

Artificial skin Dermal scaffold Human plasma Keratinocyte culture Tissue engineering 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Sara Llames
    • 1
  • Eva García
    • 1
  • Verónica García
    • 1
  • Marcela del Río
    • 2
  • Fernando Larcher
    • 2
  • Jose Luis Jorcano
    • 2
  • Eva López
    • 3
  • Purificación Holguín
    • 4
  • Francisca Miralles
    • 5
  • Jesús Otero
    • 6
  • Alvaro Meana
    • 1
    • 7
    Email author
  1. 1.CCSTAsturias
  2. 2.CIEMATMadrid
  3. 3.Plastic surgeryHospital Universitario la FeValencia
  4. 4.Plastic surgeryHospital Universitario de GetafeMadrid
  5. 5.Plastic surgeryHospital Virgen del RocíoSevilla
  6. 6.Transplant coordinatorHospital Central de AsturiasSpain
  7. 7.Centro Comunitario de Sangre y Tejidos del Principado de AsturiasOviedoSpain

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