Cardiovascular Drugs and Therapy

, Volume 24, Issue 1, pp 17–24

Aspirin Inhibits Fractalkine Expression in Atherosclerotic Plaques and Reduces Atherosclerosis in ApoE Gene Knockout Mice

Article

Abstract

Objective

To determine the fractalkine expression in the aorta of ApoE −/− mice and the effect of high-dose aspirin intervention on fractalkine expression and atherosclerotic lesion formation.

Methods

Twenty-one male ApoE gene knockout mice were randomized into three groups to receive either placebo in addition to normal mice chow (n = 7), placebo in addition to a high-fat diet (n = 7), or aspirin (58 mg/kg/d) in addition to a high-fat diet (n = 7). After 12 weeks of study, the mice were euthanized and serum cholesterol, thromboxane B2, and \( 6 - {\text{keto}} - {\text{PG}}{{\text{F}}_{1\alpha }} \) were examined. Fractalkine and cyclooxygenase expression in aorta were measured and the atherosclerotic lesion analyzed.

Results

Pathology image analysis showed that the atherosclerotic plaque was the most extensive in the high-fat diet group while the addition of aspirin greatly reduced the severity of the plaque. Both RT-PCR analysis and immunohistochemical analysis showed that fractalkine expression was the strongest in the high-fat diet group and was significantly decreased by aspirin treatment. Serum thromboxane B2 was lowered by aspirin while \( 6 - {\text{keto}} - {\text{PG}}{{\text{F}}_{1\alpha }} \) and cholesterol remained unchanged.

Conclusions

The results of our study indicate that high dose aspirin can improve the atherosclerotic lesion and suppress the fractalkine expression in murine aorta.

Key words

Aspirin Atherosclerosis Fractalkine Cyclooxygenase 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hong Liu
    • 1
  • Deqian Jiang
    • 2
  • Shebing Zhang
    • 3
  • Baiqing Ou
    • 4
  1. 1.Department of GeriatricsThe Second Xiangya Hospital of Central South UniversityChangshaChina
  2. 2.Department of CardiologyThe Second Xiangya Hospital of Central South UniversityChangshaChina
  3. 3.Department of CardiologyThe Affiliated Yue Bei People’s Hospital of Shantou University Medical CollegeShaoguanChina
  4. 4.Department of CardiologyThe Hunan People’s HospitalChangshaChina

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