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Caffeinated Coffee Blunts the Myocardial Protective Effects of Statins against Ischemia–Reperfusion Injury in the Rat

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Abstract

Purpose

We asked whether caffeinated coffee (CC) blunts the infarct size (IS)-limiting effects of atorvastatin (ATV).

Background

Adenosine receptor activation is essential for mediating the IS-limiting effects of statins. Caffeine is a nonspecific adenosine receptor blocker, and thus drinking CC may block the myocardial protective effects of statins.

Methods

Rat received 3-day ATV (10 mg/kg/day) or water by oral gavage once daily. Drinking water was replaced by water + sugar (7.5 g/100 ml), CC with sugar, or decaffeinated coffee (DC) with sugar. On the 4th day, rats were anesthetized and underwent 30 min of coronary artery occlusion and 4 h reperfusion. Area at risk was assessed by blue dye and infarct size by TTC.

Results

Body weight and area at risk was comparable among groups. IS was 25.1 ± 3.9% of the area at risk in the control group. In rats not receiving ATV, CC (25.5 ± 3.1%) and DC (34.0 ± 2.8%) did not affect IS. IS was significantly reduced by ATV in the water + sugar (11.7 ± 0.7%, p = 0.015) and DC (11.5 ± 1.0%; p < 0.001) groups, but not in the CC group (32.3 ± 3.0%; p = 0.719). ATV increased myocardial levels of Ser-473 phosphorylated Akt in the water + sugar and DC groups, but not in the CC group.

Conclusions

CC, but not DC, abrogated the IS-limiting effects of ATV by blocking the adenosine receptors and preventing the phosphorylation of Akt. CC did not affect IS in rats not receiving ATV.

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Authors and Affiliations

  1. The Division of Cardiology, University of Texas Medical Branch, 5.106 John Sealy Annex, 301 University Blvd., Galveston, TX, 77555-0553, USA

    Yumei Ye, Yu Lin & Yochai Birnbaum

  2. The Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA

    Yumei Ye, Regino J. Perez-Polo & Yochai Birnbaum

  3. The Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA

    Ghassan H. Abu Said & Saraswathy Manickavasagam

  4. The Department of Neuroscience & Cell Biology, University of Texas Medical Branch, Galveston, TX, USA

    Michael G. Hughes & David J. McAdoo

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  1. Yumei Ye
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  2. Ghassan H. Abu Said
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  3. Yu Lin
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  4. Saraswathy Manickavasagam
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  5. Michael G. Hughes
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  6. David J. McAdoo
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  7. Regino J. Perez-Polo
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  8. Yochai Birnbaum
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Corresponding author

Correspondence to Yochai Birnbaum.

Additional information

Yumei Ye and Ghassan H. Abu Said equally contributed to the manuscript.

Funding Sources: The Edward D. and Sally M. Futch Endowment of the Division of Cardiology, UTMB, Galveston, Texas.

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Ye, Y., Abu Said, G.H., Lin, Y. et al. Caffeinated Coffee Blunts the Myocardial Protective Effects of Statins against Ischemia–Reperfusion Injury in the Rat. Cardiovasc Drugs Ther 22, 275–282 (2008). https://doi.org/10.1007/s10557-008-6105-z

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  • DOI: https://doi.org/10.1007/s10557-008-6105-z

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