Cardiovascular Drugs and Therapy

, Volume 21, Issue 4, pp 227–233

Necrostatin: A Potentially Novel Cardioprotective Agent?

  • Christopher C. T. Smith
  • Sean M. Davidson
  • Shiang Y. Lim
  • James C. Simpkin
  • John S. Hothersall
  • Derek M. Yellon
Article

DOI: 10.1007/s10557-007-6035-1

Cite this article as:
Smith, C.C.T., Davidson, S.M., Lim, S.Y. et al. Cardiovasc Drugs Ther (2007) 21: 227. doi:10.1007/s10557-007-6035-1

Abstract

Background

Necrostatin-1 (Nec-1), a small tryptophan-based molecule, was recently reported to protect the cerebral cortex against ischemia-reperfusion (I/R) injury. We investigated the actions of Nec-1 and its so-called inactive analog, Nec-1i, in the setting of myocardial I/R injury.

Materials and methods

The actions of Nec-1 and Nec-1i were examined in cultured C2C12 and H9c2 myocytes, cardiomyocytes isolated from male Sprague–Dawley rats, Langendorff isolated perfused C57Bl/6J mouse hearts and an in vivo open-chest C57Bl/6J mouse heart model.

Results

Nec-1 at 30 μM and 100 μM (but not 100 μM Nec-1i) reduced peroxide-induced cell death in C2C12 cells from 51.2 ± 1.1% (control) to 26.3 ± 2.9% (p < 0.01 vs control) and 17.8 ± 0.9% (p < 0.001), respectively. With H9c2 cells cell death was also reduced from 73.0 ± 0.4% (control) to 56.7 ± 0% (30 μM Nec-1, p < 0.05) and 45.4 ± 3.3% (100 μM Nec-1, p < 0.01). In the isolated perfused heart Nec-1 (30 μM) reduced infarct size (calculated as a percentage of the risk area) from 48.0 ± 2.0% (control) to 32.1 ± 5.4% (p < 0.05). Nec-1i (30 μM) also reduced infarct size (32.9 ± 5.1%, p < 0.05). In anesthetized C57Bl/6J mice Nec-1 (1.65 mg/kg), given intraperitoneally to coincide with reperfusion following left anterior descending artery ligation (30 min), also reduced infarct size from 45.3 ± 5.1% (control) to 26.6 ± 4.0% (p < 0.05), whilst Nec-1i (1.74 mg/kg) was ineffective (37.8 ± 6.0%). Stimulus-induced opening of the mitochondrial permeability transition pore (MPTP) in rat cardiomyocytes, as reflected by the time until mitochondrial depolarisation, was unaffected by Nec-1 or Nec-1i at 30 μM but increased at 100 μM i.e. 91% (p < 0.05 vs control) and 152% (p < 0.001) for Nec-1 and Nec-1i, respectively.

Conclusion

This is the first study to demonstrate that necrostatins inhibit myocardial cell death and reduce infarct size, possibly via a mechanism independent of the MPTP.

Key words

necrostatin cardioprotection infarct size mitochondrial permeability transition pore 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Christopher C. T. Smith
    • 1
  • Sean M. Davidson
    • 1
  • Shiang Y. Lim
    • 1
  • James C. Simpkin
    • 1
  • John S. Hothersall
    • 1
  • Derek M. Yellon
    • 1
  1. 1.The Hatter Cardiovascular InstituteUniversity College London Hospital and Medical SchoolLondonUK

Personalised recommendations