The International Journal of Cardiovascular Imaging

, Volume 20, Issue 6, pp 593–598

Non-invasive analysis of myoblast transplants in rodent cardiac muscle

  • Kevin S. Cahill
  • Sean Germain
  • Barry J. Byrne
  • Glenn A. Walter
Article

DOI: 10.1007/s10554-004-3902-8

Cite this article as:
Cahill, K.S., Germain, S., Byrne, B.J. et al. Int J Cardiovasc Imaging (2004) 20: 593. doi:10.1007/s10554-004-3902-8

Abstract

Background: Magnetic resonance imaging (MRI) of magnetically labeled stem cells is a non-invasive approach that can provide images with high spatial resolution. We evaluated the ability of a commercially available, Food and Drug Administration (FDA) approved contrast agent to allow the monitoring of myoblast transplants in the rodent heart. Methods and Results: Primary rat myoblasts were efficiently labeled by incubation with ferumoxide–polycation complexes and labeled cells retained their normal capacity to generate mature myotubes. Intra-cellular iron-oxide accumulation resulted in MRI contrast changes, allowing for three-dimensional, non-invasive detection of labeled cells in the rodent myocardium. Histological analysis of hearts injected with labeled myoblasts or control, non-viable myoblasts revealed that areas of MRI contrast changes corresponded to iron contained within engrafted myotubes and scavenger cells up to two months post-injection. Conclusions: The high sensitivity of MR imaging will allow for non-invasive studies of cardiac stem cell migration and homing. Additional techniques are in development to non-invasively determine stem cell engraftment rates, viability and differentiation.

Keywords

cells heart MRI transplantation 

Abbreviations

AK

arginine kinase

C

Celsius

DMEM

Dulbecco’s modified eagle’s medium

FBS

fetal bovine serum

FDA

food and drug administration

MRI

magnetic resonance imaging

PBS

phosphate buffered saline

PLL

poly-l-lysine

SPIO

superparamagnetic iron oxide

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Kevin S. Cahill
    • 1
  • Sean Germain
    • 1
  • Barry J. Byrne
    • 1
  • Glenn A. Walter
    • 2
  1. 1.Powell Gene Therapy CenterUniversity of FloridaGainesvilleUSA
  2. 2.University of FloridaUSA

Personalised recommendations