Cancer Causes & Control

, Volume 25, Issue 4, pp 461–471 | Cite as

Human papillomavirus (HPV) 16 and the prognosis of head and neck cancer in a geographical region with a low prevalence of HPV infection

  • Rossana Verónica Mendoza López
  • José Eduardo Levi
  • José Eluf-Neto
  • Rosalina Jorge Koifman
  • Sergio Koifman
  • Maria Paula Curado
  • Pedro Michaluart-Junior
  • David Livingstone Alves Figueiredo
  • Fabiano Pinto Saggioro
  • Marcos Brasilino de Carvalho
  • Luiz Paulo Kowalski
  • Márcio Abrahão
  • Francisco de Góis-Filho
  • Eloiza Helena Tajara
  • Tim Waterboer
  • Paolo Boffetta
  • Paul Brennan
  • Victor Wünsch-Filho
Original paper

Abstract

Background

The role of human papillomavirus (HPV) on head and neck squamous cell carcinoma (HNSCC) survival in regions with low HPV prevalence is not yet clear. We evaluated the HPV16 infection on survival of HNSCC Brazilian patient series.

Methods

This cohort comprised 1,093 HNSCC cases recruited from 1998 to 2008 in four Brazilian cities and followed up until June 2009. HPV16 antibodies were analyzed by multiplex Luminex assay. In a subset of 398 fresh frozen or paraffin blocks of HNSCC specimens, we analyzed for HPV16 DNA by L1 generic primer polymerase chain reaction. HNSCC survival according to HPV16 antibodies was evaluated through Kaplan–Meier method and Cox regression.

Results

Prevalence of HPV16 E6 and E6/E7 antibodies was higher in oropharyngeal cancer than in other head and neck tumor sites. HPV16 DNA positive in tumor tissue was also higher in the oropharynx. Seropositivity for HPV16 E6 antibodies was correlated with improved HNSCC survival and oropharyngeal cancer. The presence of HPV16 E6/E7 antibodies was correlated with improved HNSCC survival and oropharyngeal cancer survival. The death risk of oropharyngeal squamous cell carcinoma patients HPV16 E6/E7 antibodies positive was 78 % lower than to those who test negative.

Conclusion

Oropharyngeal squamous cell carcinoma is less aggressive in the HPV16 E6/E7 positive serology patients. HPV16 E6/E7 antibody is a clinically sensible surrogate prognostic marker of oropharyngeal squamous cell carcinoma.

Keywords

Human papillomavirus Serology Prognosis Head and neck cancer Prevalence 

Notes

Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [10/50733-6; 09/52031-1 and 04/12054-9] and by the European Commission [IC18-CT97-0222].

Conflict of interest

None declared.

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Rossana Verónica Mendoza López
    • 1
    • 2
  • José Eduardo Levi
    • 3
  • José Eluf-Neto
    • 4
  • Rosalina Jorge Koifman
    • 5
  • Sergio Koifman
    • 5
  • Maria Paula Curado
    • 6
    • 7
  • Pedro Michaluart-Junior
    • 8
  • David Livingstone Alves Figueiredo
    • 9
  • Fabiano Pinto Saggioro
    • 9
  • Marcos Brasilino de Carvalho
    • 10
  • Luiz Paulo Kowalski
    • 11
  • Márcio Abrahão
    • 12
  • Francisco de Góis-Filho
    • 13
  • Eloiza Helena Tajara
    • 14
  • Tim Waterboer
    • 15
  • Paolo Boffetta
    • 7
    • 16
  • Paul Brennan
    • 17
  • Victor Wünsch-Filho
    • 1
  1. 1.Faculdade de Saúde PúblicaUniversidade de São PauloSão PauloBrazil
  2. 2.Instituto de Ensino e PesquisaHospital de Câncer de BarretosBarretosBrazil
  3. 3.Instituto de Medicina TropicalUniversidade de São PauloSão PauloBrazil
  4. 4.Faculdade de MedicinaUniversidade de São PauloSão PauloBrazil
  5. 5.Escola Nacional de Saúde PúblicaFundação Oswaldo CruzRio de JaneiroBrazil
  6. 6.Hospital Araujo JorgeGoiâniaBrazil
  7. 7.International Prevention Research InstituteLyonFrance
  8. 8.Hospital das Clínicas, Faculdade de MedicinaUniversidade de São PauloSão PauloBrazil
  9. 9.Hospital das Clínicas, Faculdade de Medicina de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil
  10. 10.Hospital HeliópolisSão PauloBrazil
  11. 11.Hospital do Câncer A.C. CamargoSão PauloBrazil
  12. 12.Hospital São PauloUniversidade Federal de São PauloSão PauloBrazil
  13. 13.Instituto do Câncer Arnaldo Viera de CarvalhoSão PauloBrazil
  14. 14.Faculdade de Medicina de São José do Rio PretoSão PauloBrazil
  15. 15.German Cancer Research Center (DKFZ)HeidelbergGermany
  16. 16.The Tisch Cancer InstituteMount Sinai School of MedicineNew YorkUSA
  17. 17.International Agency for Research on CancerLyonFrance

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