Cancer Causes & Control

, Volume 21, Issue 5, pp 737–743 | Cite as

Human papillomavirus is not associated with colorectal cancer in a large international study

  • Michele C. Gornick
  • Xavier Castellsague
  • Gloria Sanchez
  • Thomas J. Giordano
  • Michelle Vinco
  • Joel K. Greenson
  • Gabriel Capella
  • Leon Raskin
  • Gad Rennert
  • Stephen B. Gruber
  • Victor Moreno
Original paper

Abstract

Objective of the study

Recent publications have reported an association between colon cancer and human papillomaviruses (HPV), suggesting that HPV infection of the colonic mucosa may contribute to the development of colorectal cancer.

Methods

The GP5+/GP6+ PCR reverse line blot method was used for detection of 37 types of human papillomavirus (HPV) in DNA from paraffin-embedded or frozen tissues from patients with colorectal cancer (n = 279) and normal adjacent tissue (n = 30) in three different study populations, including samples from the United States (n = 73), Israel (n = 106) and Spain (n = 100). Additionally, SPF10 PCR was run on all samples (n = 279) and the Innogenetics INNO-LiPA assay was performed on a subset of samples (n = 15).

Results

All samples were negative for all types of HPV using both the GP5+/GP6+ PCR reverse line blot method and the SPF10 INNO-LiPA method.

Conclusions

We conclude that HPV types associated with malignant transformation do not meaningfully contribute to adenocarcinoma of the colon.

Keywords

Human papillomavirus Colorectal cancer International study 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Michele C. Gornick
    • 1
  • Xavier Castellsague
    • 2
  • Gloria Sanchez
    • 3
  • Thomas J. Giordano
    • 4
  • Michelle Vinco
    • 4
  • Joel K. Greenson
    • 4
  • Gabriel Capella
    • 2
  • Leon Raskin
    • 5
  • Gad Rennert
    • 6
  • Stephen B. Gruber
    • 1
    • 5
    • 7
  • Victor Moreno
    • 2
    • 8
  1. 1.Department of Human Genetics, School of MedicineUniversity of MichiganAnn ArborUSA
  2. 2.IDIBELL-Institut Català d’Oncologia (ICO), CIBER-ESP, L’Hospitalet de LlobregatBarcelonaSpain
  3. 3.University of AntioquiaMedellinColumbia
  4. 4.Department of Pathology, School of MedicineUniversity of MichiganAnn ArborUSA
  5. 5.Department of Internal Medicine, School of MedicineUniversity of MichiganAnn ArborUSA
  6. 6.Department of Community Medicine and Epidemiology, Carmel Medical Center, and B. Rappaport Faculty of MedicineTechnion-Israel Institute of Technology and Clalit Health Service (CHS) National Israeli Cancer Control CenterHaifaIsrael
  7. 7.Department of Epidemiology, School of Public HealthUniversity of MichiganAnn ArborUSA
  8. 8.Department of Clinical SciencesUniversity of BarcelonaBarcelonaSpain

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