Cancer Causes & Control

, Volume 20, Issue 9, pp 1739–1751 | Cite as

Association of common polymorphisms in IL10, and in other genes related to inflammatory response and obesity with colorectal cancer

  • Konstantinos K. Tsilidis
  • Kathy J. Helzlsouer
  • Michael W. Smith
  • Victoriya Grinberg
  • Judith Hoffman-Bolton
  • Sandra L. Clipp
  • Kala Visvanathan
  • Elizabeth A. Platz
Original paper


Objective and methods

The association of 17 candidate single nucleotide polymorphisms (SNPs) in IL10 and other immune response genes (CRP, TLR4, IL6, IL1B, IL8, TNF, RNASEL) and genes related to obesity (PPARG, TCF7L2, ADIPOQ, LEP) with colorectal cancer was investigated. Haplotype tagging SNPs were chosen for IL10, CRP, and TLR4. Incident colorectal cancer cases (n = 208) and matched controls (n = 381) were identified between baseline in 1989 and 2003 among participants in the CLUE II cohort. Odds ratios (OR) and 95% confidence intervals (95% CI) were estimated using conditional logistic regression.


Compared with the AA genotype at the candidate IL10-1082 locus (rs1800896), carrying one (OR, 0.79; 95% CI, 0.53–1.18) or two (OR, 0.58; 95% CI, 0.35–0.95) G alleles, a known higher producer of the anti-inflammatory cytokine IL-10, was associated with lower risk of colorectal cancer (p trend = 0.03). Statistically significant associations with colorectal cancer were observed for three tagSNPs in IL10 (rs1800890, rs3024496, rs3024498) and one common haplotype, but these associations were due to high linkage disequilibrium with IL10-1082. Two CRP haplotypes (global p = 0.04) and TLR4 tagSNPs (rs7873784, rs11536891), but not TLR4 haplotypes, were associated with colorectal cancer.


Our study suggests that polymorphisms in IL10, and also possibly in CRP and other genes related to immune response or obesity may be associated with colorectal cancer.


Inflammation Obesity Colorectal cancer Genetic epidemiology Prospective study 



We appreciate the continued efforts of the staff members at the Johns Hopkins George W. Comstock Center for Public Health Research and Prevention in the conduct of the CLUE II study.

Financial support

This research was supported by a grant from the American Institute for Cancer Research. Konstantinos Tsilidis was funded by a J. William Fulbright grant and a scholarship from the Hellenic State Scholarships Foundation.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Konstantinos K. Tsilidis
    • 1
  • Kathy J. Helzlsouer
    • 1
    • 2
    • 3
    • 4
  • Michael W. Smith
    • 5
  • Victoriya Grinberg
    • 6
  • Judith Hoffman-Bolton
    • 1
    • 3
  • Sandra L. Clipp
    • 1
    • 3
  • Kala Visvanathan
    • 1
    • 4
  • Elizabeth A. Platz
    • 1
    • 4
  1. 1.Department of EpidemiologyJohns Hopkins Bloomberg School of Public HealthBaltimoreUSA
  2. 2.Prevention and Research Center, Weinberg Center for Women’s Health and MedicineMercy Medical CenterBaltimoreUSA
  3. 3.George W. Comstock Center for Public Health Research and PreventionJohns Hopkins Bloomberg School of Public HealthHagerstownUSA
  4. 4.The Sidney Kimmel Comprehensive Cancer CenterJohns Hopkins Medical InstitutionsBaltimoreUSA
  5. 5.Genetics and Genomics, Advanced Technology ProgramSAIC-Frederick, Inc., NCI-FrederickFrederickUSA
  6. 6.Laboratory of Molecular Technology, Advanced Technology ProgramSAIC-Frederick, Inc., NCI-FrederickFrederickUSA

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