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Genomic profile of breast sarcomas: a comparison with malignant phyllodes tumours

  • Sue Zann Lim
  • Cedric Chuan Young Ng
  • Vikneswari Rajasegaran
  • Peiyong Guan
  • Sathiyamoorthy Selvarajan
  • Aye Aye Thike
  • Nur Diyana Binte Md Nasir
  • Valerie Cui Yun Koh
  • Benita Kiat Tee Tan
  • Kong Wee Ong
  • Bin Tean Teh
  • Puay Hoon Tan
Preclinical study

Abstract

Purpose

We aimed to investigate the genomic profile of breast sarcomas (BS) and compare with that of malignant phyllodes tumours (MPT).

Methods

DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) specimens from 17 cases of BS diagnosed at Singapore General Hospital from January 1991 to December 2014. Targeted deep sequencing and copy number variation (CNV) analysis on 16 genes, which included recurrently mutated genes in phyllodes tumours and genes associated with breast cancer, were performed on these samples. Genetic alterations (GA) observed were summarised and analysed.

Results

Nine cases met the quality control requirements for both targeted deep sequencing and CNV analysis. Three (33.33%) were angiosarcomas and 6 (66.67%) were non-angiosarcomas. In the non-angiosarcoma group, 83.33% (n = 5) of the patients had GA in the TERT gene. The other commonly mutated genes in this group of tumours were MED12 (n = 4, 66.67%), BCOR (n = 4, 66.67%), KMT2D (n = 3, 50%), FLNA (n = 3, 50%) and NF1 (n = 3, 50%). In contrast, none of the angiosarcomas had mutations or copy number alterations in TERT, MED12, BCOR, FLNA or NF1. Eighty percent of patients with GA in TERT (n = 5) had concurrent mutations in MED12. Sixty percent (n = 3) of these cases also demonstrated GA in NF1, PIK3CA or EGFR which are known cancer driver genes.

Conclusions

The non-angiosarcoma group of BS was found to share similar GA as those described for MPT, which may suggest a common origin and support their consideration as a similar group of tumours with regard to management and prognostication.

Keywords

Mesenchymal Genomic profile TERT MED12 NF1 

Notes

Acknowledgements

This work was supported by funding from the Singapore General Hospital Research Grant 2016.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional research board.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Sue Zann Lim
    • 1
  • Cedric Chuan Young Ng
    • 2
    • 3
  • Vikneswari Rajasegaran
    • 2
    • 3
  • Peiyong Guan
    • 4
  • Sathiyamoorthy Selvarajan
    • 5
  • Aye Aye Thike
    • 5
  • Nur Diyana Binte Md Nasir
    • 5
  • Valerie Cui Yun Koh
    • 5
  • Benita Kiat Tee Tan
    • 1
  • Kong Wee Ong
    • 1
  • Bin Tean Teh
    • 2
    • 3
    • 6
    • 7
  • Puay Hoon Tan
    • 8
  1. 1.SingHealth Duke-National University of Singapore Breast CentreSingaporeSingapore
  2. 2.Laboratory of Cancer EpigenomeNational Cancer Centre SingaporeSingaporeSingapore
  3. 3.Division of Cancer and Stem Cell BiologyDuke-National University of Singapore Medical SchoolSingaporeSingapore
  4. 4.Integrated Biostatistics and Bioinformatics ProgrammeDuke-National University of Singapore Medical SchoolSingaporeSingapore
  5. 5.Department of Anatomical PathologySingapore General HospitalSingaporeSingapore
  6. 6.Cancer Science Institute of SingaporeNational University of SingaporeSingaporeSingapore
  7. 7.Institute of Molecular and Cellular BiologySingaporeSingapore
  8. 8.Division of PathologySingapore General HospitalSingaporeSingapore

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