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Breast Cancer Research and Treatment

, Volume 174, Issue 1, pp 129–141 | Cite as

Plasma exosomes stimulate breast cancer metastasis through surface interactions and activation of FAK signaling

  • Tatiana Shtam
  • Stanislav Naryzhny
  • Roman Samsonov
  • David Karasik
  • Igor Mizgirev
  • Artur Kopylov
  • Elena Petrenko
  • Yana Zabrodskaya
  • Roman Kamyshinsky
  • Daniil Nikitin
  • Maxim Sorokin
  • Anton Buzdin
  • Hava Gil-HennEmail author
  • Anastasia MalekEmail author
Preclinical study
  • 322 Downloads

Abstract

Purpose

The interaction between malignant cells and surrounding healthy tissues is a critical factor in the metastatic progression of breast cancer (BC). Extracellular vesicles, especially exosomes, are known to be involved in inter-cellular communication during cancer progression. In the study presented herein, we aimed to evaluate the role of circulating plasma exosomes in the metastatic dissemination of BC and to investigate the underlying molecular mechanisms of this phenomenon.

Methods

Exosomes isolated from plasma of healthy female donors were applied in various concentrations into the medium of MDA-MB-231 and MCF-7 cell lines. Motility and invasive properties of BC cells were examined by random migration and Transwell invasion assays, and the effect of plasma exosomes on the metastatic dissemination of BC cells was demonstrated in an in vivo zebrafish model. To reveal the molecular mechanism of interaction between plasma exosomes and BC cells, a comparison between un-treated and enzymatically modified exosomes was performed, followed by mass spectrometry, gene ontology, and pathway analysis.

Results

Plasma exosomes stimulated the adhesive properties, two-dimensional random migration, and transwell invasion of BC cells in vitro as well as their in vivo metastatic dissemination in a dose-dependent manner. This stimulatory effect was mediated by interactions of surface exosome proteins with BC cells and consequent activation of focal adhesion kinase (FAK) signaling in the tumor cells.

Conclusions

Plasma exosomes have a potency to stimulate the metastasis-promoting properties of BC cells. This pro-metastatic property of normal plasma exosomes may have impact on the course of the disease and on its prognosis.

Keywords

Breast cancer Metastasis Exosomes Surface interaction Mass spectrometry FAK signaling 

Notes

Acknowledgements

This study was supported by RFBR (grant numbers 15-54-12380 and 18-015-00289), by Russian Science Foundation (grant number 17-14-01416), by the Ministry of Education and Science of the Russian Federation (ID RFMEFI62117 × 0017) (to Anastasia Malek), as well as by the Israel Cancer Association and Estee Lauder Companies (grant number 20180089), by the Israel Cancer Research Foundation (grant number 17-902-AG), and by the Israel Science Foundation (grant number 1462/17) (to Hava Gil-Henn).

Compliance with ethical standards

Conflict of interest

The authors declared no conflicts of interest.

Ethical approval

The design of study has been approved by Ethical Committee of N.N. Petrov National Medical Research Center of Oncology. All procedures were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki Declaration. All applicable international, national, and institutional guidelines for the care and use of animals were followed.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

10549_2018_5043_MOESM1_ESM.xlsx (181 kb)
Supplementary material 1 (XLSX 180 KB)
10549_2018_5043_MOESM2_ESM.tif (27.6 mb)
Supplementary material 2 (TIF 28278 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Tatiana Shtam
    • 1
    • 2
    • 3
  • Stanislav Naryzhny
    • 3
    • 4
  • Roman Samsonov
    • 1
    • 2
  • David Karasik
    • 5
  • Igor Mizgirev
    • 1
  • Artur Kopylov
    • 4
  • Elena Petrenko
    • 4
  • Yana Zabrodskaya
    • 3
    • 6
  • Roman Kamyshinsky
    • 7
  • Daniil Nikitin
    • 8
    • 9
  • Maxim Sorokin
    • 9
    • 10
  • Anton Buzdin
    • 8
    • 9
    • 10
  • Hava Gil-Henn
    • 5
    Email author
  • Anastasia Malek
    • 1
    • 2
    Email author
  1. 1.N.N. Petrov National Medical Research Center of OncologySt.-PetersburgRussia
  2. 2.Oncosystem Ltd.MoscowRussia
  3. 3.Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Center “Kurchatov Institute”GatchinaRussia
  4. 4.Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical SciencesMoscowRussia
  5. 5.The Azrieli Faculty of MedicineBar-Ilan UniversitySafedIsrael
  6. 6.Smorodintsev Research Institute of InfluenzaMinistry of Healthcare of the Russian FederationSt.-PetersburgRussia
  7. 7.National Research Center “Kurchatov Institute”MoscowRussia
  8. 8.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryMoscowRussia
  9. 9.Omics Way Corp.WalnutUSA
  10. 10.I.M. Sechenov First Moscow State Medical University (Sechenov University)MoscowRussia

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