Mutational analysis of candidate genes in Israeli male breast cancer cases
To define the mutational spectrum of several candidate gene mutations in Israeli male breast cancer cases.
MBC cases counselled at the Oncogenetics unit, Sheba Medical Center from January 1998 to June 2017 were included. Relevant clinical and oncological data and cancer phenotype were retrieved. All participants were genotyped for the predominant Jewish BRCA1 and BRCA2 germline mutations using a chip-based assay. Those who tested negative were further genotyped for three recurring mutations in CHEK2 (c.1100delC, p.S428F, p.I157T), and single mutations in the FANCM (c.5791C>T), and RAD51D (c.556C>T) genes, by direct sequencing. The ethics committee approved the study.
Overall, 61 MBC were identified and genotyped, 41 (67.2%) were Ashkenazim, age at diagnosis was 58.1 ± 12.6 years, and 31 (50.8%) had a family history of cancer. Of genotyped individuals, one (1.6%) harboured the 185delAG* BRCA1 mutation, 7 (11.4%) the 6174delT*BRCA2 mutation and 2 (3.2%) other recurring mutations in BRCA2 (overall 10/61–16.4% BRCA1/BRCA2 mutation carriers). Of BRCA-negative cases, 3/51 (5.9%) carried the p.S428F *CHEK2 mutation. None was a carrier of the other genotyped mutations in CHEK2, FANCM or RAD51D.
BRCA1, BRCA2 and CHEK2 germline mutations contribute to inherited predisposition to MBC in Israel.
KeywordsMale breast cancer Inherited predisposition to cancer BRCA1 BRCA2 CHEK2
This work was carried out in partial fulfilment for the degree of MD to Hila Korach, at the Sackler School of Medicine, Tel-Aviv University, Tel- Aviv, Israel. This study was in part funded by the Grant top the Israeli consortium of inherited breast and ovarian cancer.
Compliance with ethical standards
Conflict of interest
All authors confirm that they have no conflict of interest with the data reported herein.
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