Breast Cancer Research and Treatment

, Volume 156, Issue 1, pp 65–72 | Cite as

MSCs and inflammation: new insights into the potential association between ALCL and breast implants

  • M. Orciani
  • G. Sorgentoni
  • M. Torresetti
  • Roberto Di PrimioEmail author
  • G. Di Benedetto
Preclinical Study


Possible association between anaplastic large cell lymphoma (ALCL) and breast implants has been suggested. In this context, formation of the periprosthetic capsule has been reported as a cause of inflammation, which plays a key role in tumor onset. Tumors take advantage of inflammation to influence and interfere with the host immune response by secreting multiple factors, and their onset and survival is in turn affected by the paracrine effects from mesenchymal stem cells (MSCs). In this study, we tried to clarify how inflammation can modify the immunobiology and the exerted paracrine effect of MSCs. MSCs derived from both inflamed (I-MSCs) and control (C-MSCs) tissues were isolated and co-cultured with an ALCL cell line. Proliferation rate and the expression of selected cytokines were tested. I-MSCs secrete higher levels of cytokine related to chronic inflammation than C-MSCs. After co-cultures with KI-JK cells, C- and I-MSCs show the same variation in the cytokine expression, with an increase of IL2, IL4, IL5, IL10, IL13, TNF-α, TGF-β, and G-CSF. Proliferation of ALCL cells was not influenced by co-cultures. Our results state that (i) inflamed microenvironment affects the immunobiology of MSCs modifying the profile of the expressed cytokines, and (ii) the paracrine effects exerted by MSCs on ALCL cells are not influenced by inflammation. Moreover, it seems that ALCL cells are able to manipulate MSCs’ immunoregulatory properties to evade the host immune control. Nevertheless, this ability is not associated with inflammation and the question about BIA-ALCL is not proved by our experiments.


ALCL MSCs Breast implants Paracrine effects 



This work was supported by the Grants FIRB-RBAP1153LS_004 and PRIN 201098WFZ2_006 from Ministero dell’Istruzione, dell’Università e della Ricerca, Rome, Italy.

Compliance with ethical standards

Conflict of interest

None of the authors have a financial interest in any of the products, devices, or drugs mentioned in this manuscript.

Ethical approval

The study and the patient enrollment were approved by the Marche Polytechnic University Ethical Commitee and were conducted in accordance with the Declaration of Helsinki.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • M. Orciani
    • 1
  • G. Sorgentoni
    • 1
  • M. Torresetti
    • 2
  • Roberto Di Primio
    • 1
    Email author
  • G. Di Benedetto
    • 2
  1. 1.Department of Clinical and Molecular Sciences – HistologyUniversità Politecnica delle MarcheAnconaItaly
  2. 2.Department of Experimental and Clinical Medicine – Clinic of Plastic and Reconstructive SurgeryUniversità Politecnica delle MarcheAnconaItaly

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