Breast Cancer Research and Treatment

, Volume 134, Issue 2, pp 899–901 | Cite as

A bias in genotyping the miR-27a rs895819 and rs11671784 variants

  • Rongxi Yang
  • Barbara Burwinkel
Letter to the Editor

To the Editor,

Due to the oncogenetic function of miR-27a, SNPs affecting this gene are frequently investigated for an influence on cancer risk. Yang et al. [1] has reported the association between the minor [G] allele of rs895819 on miR-27a and a reduced familial breast cancer risk in German population by direct sequencing. One study in Chinese Han population also observed a tumor suppressive effect of the rs895819 [G] allele in gastric cancer using MALDI-TOF MassARRAY [2].

One very recent publication has analysed the association of rs895819 with familial breast cancer risk in Italian population using TaqMan allelic discrimination assay [ 3]. However, this genotyping method appears to be inappropriate for rs895819 (chr19:13808292) due to the adjacent variant rs11671784 (chr19:13808296, which is located only 4 nucleotides distance and represents a rare variant, with the rare [T] allele frequency of 2.4 % and 1.9 % in German familial breast cancer cases and healthy controls,...


TaqMan Probe German Population Familial Breast Cancer Tumor Suppressive Effect Nucleotide Distance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Ludwig Heesen, Michelle Dick, Ying Wang and Anja Schwaeger for participating in genotyping. We are grateful to Bowang Chen for statistical analyses as well as to Sandrine Tchatchou for support. The German breast cancer samples were collected within a project funded by the Deutsche Krebshilfe (Grant Number: 107054). This study was supported by the Helmholtz society, the German Cancer Research Center (DKFZ), EU, LSHC-CT-2004-503465 and the Dietmar-Hopp Foundation.

Conflict of interest



  1. 1.
    Yang R, Schlehe B, Hemminki K, Sutter C, Bugert P, Wappenschmidt B, Volkmann J, Varon R, Weber BH, Niederacher D et al (2010) A genetic variant in the pre-miR-27a oncogene is associated with a reduced familial breast cancer risk. Breast Cancer Res Treat 121:693–702PubMedCrossRefGoogle Scholar
  2. 2.
    Zhou Y, Du WD, Chen G, Ruan J, Xu S, Zhou FS, Zuo XB, Lv ZJ, Zhang XJ (2012) Association analysis of genetic variants in microRNA networks and gastric cancer risk in a Chinese Han population. J Cancer Res Clin Oncol 138:939–945PubMedCrossRefGoogle Scholar
  3. 3.
    Catucci I, Verderio P, Pizzamiglio S, Bernard L, Dall’olio V, Sardella D, Ravagnani F, Galastri L, Barile M, Peissel B et al (2012) The SNP rs895819 in miR-27a is not associated with familial breast cancer risk in Italians. Breast Cancer Res Treat 133:805–807PubMedCrossRefGoogle Scholar
  4. 4.
    Frank B, Hemminki K, Burwinkel B (2005) A bias in genotyping the ERBB2 (HER2) Ile655Val variant. Carcinogenesis 26:1649PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC. 2012

Authors and Affiliations

  1. 1.Helmholtz-University Group Molecular EpidemiologyGerman Cancer Research Center (DKFZ)HeidelbergGermany
  2. 2.Division Molecular Biology of Breast Cancer, Department of Gynecology and ObstetricsUniversity of HeidelbergHeidelbergGermany

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