Breast Cancer Research and Treatment

, Volume 133, Issue 3, pp 969–977 | Cite as

Investigation of human JC and BK polyomaviruses in breast carcinomas

  • Mohamed Hachana
  • Khaled Amara
  • Sonia Ziadi
  • Riadh Ben Gacem
  • Sadok Korbi
  • Mounir Trimeche
Preclinical Study

Abstract

We have previously showed the presence of the simian virus 40 (SV40) and the mouse mammary tumor virus (MMTV)-like in a significant proportions of Tunisian breast carcinomas. However, to date there are no published studies concerning evaluation of the possible implication of the human polyomaviruses JC (JCV) and BK (BKV) in breast carcinomas. The presence of JCV and BKV DNA was investigated by PCR in a 123 primary breast carcinomas and matched adjacent non-tumor breast tissues. The results were correlated to clinicopathological and virological parameters. JCV T-antigen DNA was detected in 23% of breast carcinoma cases; however, all cases were negative for BKV. JCV T antigen PCR products were further confirmed as authentic JCV genome by direct sequencing. JCV was found in invasive ductal carcinomas (28/112 cases) but not in invasive lobular carcinomas (0/5) or medullary carcinomas (0/6). JCV DNA presence correlates inversely with the expression of estrogen (P = 0.022) and progesterone (P = 0.008) receptors. JCV DNA presence correlates also with “triple negative” phenotype (P = 0.021). With regard to virological data, a trend toward an inverse correlation was noted between the presence of JCV and SV40 (P = 0.06). Moreover, significant correlation was found between multiple viral infection (JCV, and/or SV40, and/or MMTV-like in the same tumor) and “triple negative” phenotype (P = 0.001) and also with p53 accumulation (P = 0.028). To the best of our knowledge, this is the first study demonstrating the presence of JCV in a subset of breast carcinomas. Also our results suggest that “triple negative” breast carcinomas are viral-related tumors.

Keywords

Breast cancer Polyomaviruses BK virus JC virus Tunisia 

Notes

Acknowledgments

This study was supported by the “Ministère de l’Enseignement Supérieur, de la Recherche Scientifique et Technologie” and the “Ministère de la Santé Publique” of Tunisia.

Conflict of interest

The authors have no conflicts of interest in relation to this article.

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Mohamed Hachana
    • 1
  • Khaled Amara
    • 1
  • Sonia Ziadi
    • 1
  • Riadh Ben Gacem
    • 1
  • Sadok Korbi
    • 1
  • Mounir Trimeche
    • 1
  1. 1.Department of PathologyFarhat Hached HospitalSousseTunisia

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