Breast Cancer Research and Treatment

, Volume 133, Issue 2, pp 583–594 | Cite as

Characterisation of amplification patterns and target genes at chromosome 11q13 in CCND1-amplified sporadic and familial breast tumours

  • Karolina Holm
  • Johan Staaf
  • Göran Jönsson
  • Johan Vallon-Christersson
  • Haukur Gunnarsson
  • Adalgeir Arason
  • Linda Magnusson
  • Rosa B. Barkardottir
  • Cecilia Hegardt
  • Markus Ringnér
  • Åke Borg
Preclinical study


Amplification of chromosomal region 11q13, containing the cell cycle regulatory gene CCND1, is frequently found in breast cancer and other malignancies. It is associated with the favourable oestrogen receptor (ER)-positive breast tumour phenotype, but also with poor prognosis and treatment failure. 11q13 spans almost 14 Mb and contains more than 200 genes and is affected by various patterns of copy number gains, suggesting complex mechanisms and selective pressure during tumour progression. In this study, we used 32 k tiling BAC array CGH to analyse 94 CCND1-amplified breast tumours from sporadic, hereditary, and familial breast cancers to fine map chromosome 11q13. A set containing 281 CCND1-non-amplified breast tumours was used for comparisons. We used gene expression data to further validate the functional effect of gene amplification. We identified six core regions covering 11q13.1-q14.1 that were amplified in different combinations. The major core contained CCND1, whereas two cores were found proximal of CCND1 and three distal. The majority of the CCND1-amplified tumours were ER-positive and classified as luminal B. Furthermore, we found that CCND1 amplification is associated with a more aggressive phenotype within histological grade 2 tumours and luminal A subtype tumours. Amplification was equally prevalent in familial and sporadic tumours, but strikingly rare in BRCA1- and BRCA2-mutated tumours. We conclude that 11q13 includes many potential target genes in addition to CCND1.


CCND1 11q13 Array comparative genomic hybridisation Breast cancer Gene amplification 



Array comparative genomic hybridisation




CCND1-amplified tumours


CCND1-non-amplified tumours


Oestrogen receptor


Fraction of genome altered


Fluorescence in situ hybridisation


Gene expression omnibus


Gene expression


Genomic identification of significant targets in cancer


Gene expression-based outcome for breast cancer online


Overall survival


Progesterone receptor


Sample adaptive threshold


Smallest region of overlap



This study was supported by grants from the Swedish Cancer Society, the Swedish Research Council, the Berta Kamprad Foundation, the Gunnar Nilsson Foundation, the King Gustaf V Jubilee Foundation, the Knut & Alice Wallenberg Foundation, the Foundation for Strategic Research, and the IngaBritt and Arne Lundberg Foundation. The SCIBLU Genomics Centre is supported by governmental funding of clinical research within the national health services (ALF) and by Lund University.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

10549_2011_1817_MOESM1_ESM.xls (108 kb)
Supplementary material 1 (XLS 109 kb)
10549_2011_1817_MOESM2_ESM.pdf (549 kb)
Supplementary material 2 (PDF 550 kb)


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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Karolina Holm
    • 1
    • 2
  • Johan Staaf
    • 1
    • 2
  • Göran Jönsson
    • 1
    • 2
  • Johan Vallon-Christersson
    • 1
    • 2
  • Haukur Gunnarsson
    • 3
  • Adalgeir Arason
    • 3
    • 4
  • Linda Magnusson
    • 5
  • Rosa B. Barkardottir
    • 3
    • 4
  • Cecilia Hegardt
    • 1
  • Markus Ringnér
    • 1
    • 2
  • Åke Borg
    • 1
    • 2
    • 6
  1. 1.Department of Oncology, Clinical SciencesLund UniversityLundSweden
  2. 2.CREATE Health Strategic Centre for Translational Cancer ResearchLund University, BMC C13LundSweden
  3. 3.Department of Pathology, Laboratory of Cell BiologyLandspitali University HospitalReykjavikIceland
  4. 4.Faculty of MedicineUniversity of IcelandReykjavikIceland
  5. 5.Department of Clinical GeneticsUniversity and Regional Laboratories, Skåne University Hospital, Lund UniversityLundSweden
  6. 6.Lund Strategic Research Center for Stem Cell Biology and Cell TherapyLund UniversityLundSweden

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