Breast Cancer Research and Treatment

, Volume 128, Issue 3, pp 647–655

Clinical significance of high focal adhesion kinase gene copy number and overexpression in invasive breast cancer

  • Cha Kyong Yom
  • Dong-Young Noh
  • Woo Ho Kim
  • Hee Sung Kim
Preclinical study
  • 216 Downloads

Abstract

Increased expression and activation of focal adhesion kinase (FAK) was reported to be an unfavorable factor in various human cancers, including breast cancer. In order to study FAK gene status as a prognostic factor, we evaluated FAK gene copy number and FAK protein expression in invasive breast cancer. Tumors from 435 patients with invasive breast cancer were evaluated for FAK gene status using fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) based on tissue array method. Survival analyses were performed using the Kaplan–Meier method in 267 patients. 42 out of 362 evaluable cases (11.6%) showed high polysomy and 22 cases (6.1%) had gene amplification by FISH. 108 out of 393 evaluable cases (27.5%) showed FAK overexpression by IHC. FAK FISH positivity was significantly associated with higher histologic grade, higher T stage, negative estrogen receptor expression, negative progesterone receptor expression and triple-negative phenotype; FAK overexpression with higher histologic grade and triple-negative phenotype. FAK overexpression was noted in 57.8% (37 of 64) of FAK FISH+ cases. The concordance of FISH and IHC results for FAK gene was observed in 74.9% (271 of 362). Survival analyses revealed the patients with FAK FISH+ breast cancer had significantly shorter overall survival and relapse-free survival compared to those with FISH− breast cancer. In node-positive breast cancer patients who received postoperative systemic treatment, the patients with FAK FISH+ showed significantly shorter 5-year survival rates. Despite of high significant concordance between the results of FISH and IHC for FAK gene in invasive breast cancer, only FAK FISH positivity was an unfavorable prognostic factor.

Keywords

Focal adhesion kinase Gene copy number Breast cancer Prognosis 

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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Cha Kyong Yom
    • 1
  • Dong-Young Noh
    • 1
  • Woo Ho Kim
    • 2
  • Hee Sung Kim
    • 3
  1. 1.Department of Surgery, Cancer Research InstituteSeoul National University College of MedicineSeoulKorea
  2. 2.Department of Pathology, Cancer Research InstituteSeoul National University College of MedicineSeoulKorea
  3. 3.Department of PathologyKEPCO Medical Foundation, Hanil General HospitalSeoulKorea

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