Breast Cancer Research and Treatment

, Volume 125, Issue 1, pp 207–214

Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk

  • Genevieve M. Monsees
  • Peter Kraft
  • Stephen J. Chanock
  • David J. Hunter
  • Jiali Han
Epidemiology

Abstract

Mistakes in DNA repair can result in sustained damage and genetic instability. We comprehensively evaluated common variants in DNA repair pathway genes for their association with postmenopausal breast cancer risk with and without respect to estrogen receptor (ER) and progesterone receptor (PR) subtypes. In this study of 1,145 prospectively ascertained breast cancer cases and 1,142 matched controls from the Nurses’ Health Study Cancer Genetic Markers of Susceptibility project, we evaluated 1,314 common genetic variants in 68 candidate genes. These variants were chosen to represent five DNA repair pathways including base excision repair, nucleotide excision repair, double-strand break repair (homologous recombination and non-homologous end-joining), direct reversal repair, and mismatch repair, along with candidate DNA polymerases, Fanconi Anemia complementation groups, and other genes relevant to DNA damage recognition and response. Main effects, pathway effects, and pair-wise interactions were evaluated using Logistic Regression, and the Admixture Maximum Likelihood (AML) and Kernel Machine tests. Eight linked loci within XRCC4 were associated with susceptibility to PR− breast cancer (main effect p-values corrected for multiple testing at the within-gene level < 0.04). These loci drove the association between the non-homologous end-joining pathway, and PR− breast cancer (AML p-value for the full pathway = 0.002; p-value when the eight loci were removed = 0.86). A Kernel machine test of no linear or quadratic effects, or pairwise interaction, yielded a p-value of 0.85. Common variation alone in DNA repair genes plays at most a small role in determining postmenopausal breast cancer risk among women of European ancestry.

Keywords

Polymorphism DNA repair Breast cancer Postmenopausal women Pathway 

Abbreviations

BER

Base excision repair

CGEMS

Cancer Genetic Markers of Susceptibility

DIRECT

Direct reversal repair

DSB

Double-strand break repair

ER

Estrogen receptor

FAN

Fanconi anemia complementation groups

HR

Homologous recombination

LD

Linkage disequilibrium

MAF

Minor allele frequency

MMR

Mismatch repair

NER

Nucleotide excision repair

NH

Non-homologous end joining

NHS

Nurses’ Health Study

PMH

Postmenopausal hormone

POL

Candidate DNA polymerases

PR

Progesterone receptor

REG

DNA damage recognition and response

SNP

Single nucleotide polymorphism

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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Genevieve M. Monsees
    • 1
    • 2
    • 3
    • 4
  • Peter Kraft
    • 1
    • 5
  • Stephen J. Chanock
    • 6
  • David J. Hunter
    • 1
    • 2
    • 7
  • Jiali Han
    • 1
    • 2
    • 8
  1. 1.Program in Molecular and Genetic Epidemiology, Department of EpidemiologyHarvard School of Public HealthBostonUSA
  2. 2.Channing Laboratory, Department of MedicineHarvard Medical SchoolBostonUSA
  3. 3.Fred Hutchinson Cancer Research CenterSeattleUSA
  4. 4.Department of EpidemiologyUniversity of WashingtonSeattleUSA
  5. 5.Department of BiostatisticsHarvard School of Public HealthBostonUSA
  6. 6.Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of HealthDepartment of Health and Human ServicesBethesdaUSA
  7. 7.Department of NutritionHarvard School of Public HealthBostonUSA
  8. 8.Department of DermatologyBrigham and Women’s HospitalBostonUSA

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