Breast Cancer Research and Treatment

, Volume 123, Issue 3, pp 757–765 | Cite as

Gene amplification in ductal carcinoma in situ of the breast

  • L. Burkhardt
  • T. J. Grob
  • I. Hermann
  • E. Burandt
  • M. Choschzick
  • F. Jänicke
  • V. Müller
  • C. Bokemeyer
  • R. Simon
  • G. Sauter
  • W. Wilczak
  • A. Lebeau
Preclinical study


Multiple different biologically and clinically relevant genes are often amplified in invasive breast cancer, including HER2, ESR1, CCND1, and MYC. So far, little is known about their role in tumor progression. To investigate their significance for tumor invasion, we compared pure ductal carcinoma in situ (DCIS) and DCIS associated with invasive cancer with regard to the amplification of these genes. Fluorescence in situ hybridization (FISH) was performed on a tissue microarray containing samples from 130 pure DCIS and 159 DCIS associated with invasive breast cancer. Of the latter patients, we analyzed the intraductal and invasive components separately. In addition, lymph node metastases of 23 patients with invasive carcinoma were included. Amplification rates of pure DCIS and DCIS associated with invasive cancer did not differ significantly (pure DCIS vs. DCIS associated with invasive cancer: HER2 22.7 vs. 24.2%, ESR1 19.0 vs. 24.1%, CCND1 10.0 vs. 14.8%, MYC 11.8 vs. 6.5%; P > 0.05). Furthermore, we observed a high concordance of the amplification status for all genes if in situ and invasive carcinoma of individual patients were compared. This applied also to the corresponding lymph node metastases. Our results indicate no significant differences between the gene amplification status of DCIS and invasive breast cancer concerning HER2, ESR1, CCND1, and MYC. Therefore, our data suggest an early role of all analyzed gene amplifications in breast cancer development but not in the initiation of invasive tumor growth.


DCIS Amplification HER2 ESR1 CCND1 MYC 



This work was supported by the Deutsche Krebshilfe e. V., Germany (KH 108059).


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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • L. Burkhardt
    • 1
  • T. J. Grob
    • 1
  • I. Hermann
    • 1
  • E. Burandt
    • 1
  • M. Choschzick
    • 1
  • F. Jänicke
    • 2
  • V. Müller
    • 2
  • C. Bokemeyer
    • 3
  • R. Simon
    • 1
  • G. Sauter
    • 1
  • W. Wilczak
    • 1
  • A. Lebeau
    • 1
  1. 1.Department of PathologyUniversity Medical Center Hamburg-EppendorfHamburgGermany
  2. 2.Department of GynecologyUniversity Medical Center Hamburg-EppendorfHamburgGermany
  3. 3.Department of Internal Medicine II, Oncology CenterUniversity Medical Center Hamburg-EppendorfHamburgGermany

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