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Breast Cancer Research and Treatment

, Volume 120, Issue 3, pp 603–612 | Cite as

The prognostic significance of PELP1 expression in invasive breast cancer with emphasis on the ER-positive luminal-like subtype

  • Hany Onsy Habashy
  • Desmond G. Powe
  • Emad A. Rakha
  • Graham Ball
  • R. Douglas Macmillan
  • Andrew R. Green
  • Ian O. Ellis
Preclinical study

Abstract

The transcription functions of oestrogen receptors (ER) are influenced by several coregulators such as PELP1 (proline, glutamate and leucine rich protein 1). The aim of the present study, which uses tissue microarrays and immunohistochemistry, is to explore the clinical and biological relevance of PELP1 protein expression in a large series of consecutive patients (1,162 patients) with invasive breast cancers with particular emphasis on its role in the ER-positive/luminal-like class of tumours. Our results showed that increased PELP1 expression is associated with tumours of larger size, higher histological grade, higher mitotic count, and with positive expression of basal cytokeratins (CK) (CK14; P = 0.018 and CK5/6; P = 0.029), P-cadherin (P = 0.002), p53 and MIB1 (P = 0.018). There was an inverse association between PELP1 expression and ER (P = 0.002), progesterone (PgR) (P = 0.004), androgen (AR) receptor (P < 0.001), and luminal CK (CK18; P = 0.027) expression. A significant association between PELP1 expression and shorter breast cancer specific survival (BCSS) (P = 0.002) and disease-free survival (DFI) (P = 0.006) was found. Multivariate Cox hazard analysis showed that PELP1 expression was an independent predictor of shorter BCSS (Hazard ratio (HR) = 1.349, P = 0.006) and shorter DFI (HR = 1.255, P = 0.011). In the ER-positive/luminal-like group (n = 768), PELP1 expression showed similar association with other clinicopathological variables and was an independent predictor of shorter DFI (HR = 1.256, P = 0.036). In conclusion, PELP1 protein expression is an independent prognostic predictor of shorter BCSS and DFI in breast cancer and its elevated expression is positively associated with markers of poor outcome. PELP1 appears to have a potential application in assessing the clinical outcome of patients with ER-positive breast cancer.

Keywords

Breast carcinoma PELP1 Oestrogen receptor Prognosis Immunohistochemistry Tissue microarray 

Notes

Acknowledgments

We thank the Ministry of Higher Education (Egypt) for funding H. O. Habashy and E. A. Rakha and Breast Cancer Campaign for funding A Green.

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Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Hany Onsy Habashy
    • 1
    • 4
  • Desmond G. Powe
    • 1
  • Emad A. Rakha
    • 1
  • Graham Ball
    • 2
  • R. Douglas Macmillan
    • 3
  • Andrew R. Green
    • 1
  • Ian O. Ellis
    • 1
  1. 1.Department of Histopathology, School of Molecular Medical Sciences, Nottingham University Hospitals NHS TrustUniversity of NottinghamNottinghamUK
  2. 2.School of Science and TechnologyNottingham Trent UniversityNottinghamUK
  3. 3.The Breast InstituteNottingham University HospitalsNottinghamUK
  4. 4.Department of Pathology, Faculty of MedicineMansoura UniversityMansouraEgypt

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