Breast Cancer Research and Treatment

, Volume 116, Issue 2, pp 329–338 | Cite as

A small subgroup of operable breast cancer patients with poor prognosis identified by quantitative real-time RT-PCR detection of mammaglobin A and trefoil factor 1 mRNA expression in bone marrow

  • Kjersti Tjensvoll
  • Bjørnar Gilje
  • Satu Oltedal
  • Victor F. Shammas
  • Jan Terje Kvaløy
  • Reino Heikkilä
  • Oddmund Nordgård
Clinical trial


Purpose The utility of three different epithelial mRNA markers to detect clinically significant, disseminated tumour cells in bone marrow (BM) was explored. Methods Mammaglobin A (hMAM), trefoil factor 1 (TFF-1) and prostate derived Ets factor (PDEF) mRNA were quantitated by real-time RT-PCR in BM samples from 192 breast cancer patients undergoing surgery (control group: 26 healthy women). Results During a median follow-up of 72 months, four of the five hMAM BM-positive and three of the seven TFF-1 BM-positive patients experienced a systemic relapse. Kaplan-Meier survival analyses demonstrated significantly shorter recurrence-free-, breast-cancer-specific- and overall survival for both hMAM and TFF-1 BM-positive patients. In contrast, PDEF mRNA quantitation did not reveal any significant differences in the survival analyses. Multivariate Cox regression demonstrated hMAM mRNA BM expression to be an independent predictor of both overall- (hazard ratio = 5.896), breast-cancer-specific- (hazard ratio = 10.208) and systemic-recurrence-free survival (hazard ratio = 14.304). TFF-1 status was related to hMAM status (P < 0.001). Conclusion Breast cancer patients with pre-operative elevated BM levels of hMAM and/or TFF-1 mRNA seem to constitute a small group of patients with a very poor prognosis.


Breast cancer Micrometastasis Mammaglobin TFF-1 PDEF Real-time PCR 



This study was partly financed by the Norwegian Cancer Society.

Supplementary material

10549_2008_204_MOESM1_ESM.pdf (55 kb)
(PDF 55 kb)


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Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Kjersti Tjensvoll
    • 1
  • Bjørnar Gilje
    • 2
  • Satu Oltedal
    • 1
  • Victor F. Shammas
    • 2
  • Jan Terje Kvaløy
    • 3
    • 4
  • Reino Heikkilä
    • 1
    • 2
  • Oddmund Nordgård
    • 1
    • 2
    • 3
  1. 1.Laboratory for Molecular BiologyStavanger University HospitalStavangerNorway
  2. 2.Department of Haematology and OncologyStavanger University HospitalStavangerNorway
  3. 3.Department of Mathematics and Natural SciencesUniversity of StavangerStavangerNorway
  4. 4.Division of Research and Human ResourcesStavanger University HospitalStavangerNorway

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