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Journal of Inherited Metabolic Disease

, Volume 36, Issue 3, pp 455–466 | Cite as

The CXCL12/CXCR4/CXCR7 ligand-receptor system regulates neuro-glio-vascular interactions and vessel growth during human brain development

  • Daniela Virgintino
  • Mariella Errede
  • Marco Rizzi
  • Francesco Girolamo
  • Maurizio Strippoli
  • Thomas Wälchli
  • David Robertson
  • Karl Frei
  • Luisa Roncali
Original Article

Abstract

This study investigates glio-vascular interactions in human fetal brain at midgestation, specifically examining the expression and immunolocalization of the CXCL12/CXCR4/CXCR7 ligand-receptor axis and its possible role in the vascular patterning of the developing brain. At midgestation, the telencephalic vesicles are characterized by well developed radial glia cells (RGCs), the first differentiated astrocytes and a basic vascular network mainly built of radial vessels. RGCs have been recognized to contribute to cerebral cortex neuro-vascular architecture and have also been demonstrated to act as a significant source of neural cells (Rakic, Brain Res 33:471–476, 1971; Malatesta et al, Development 127:5253–5263, 2000). According to our hypothesis CXCL12, a potent migration and differentiation chemokine released by RGCs, may act as a linking factor coordinating neuroblast migration with vessel growth and patterning through the activation of different ligand/receptor axes. The obtained results support this hypothesis showing that together with CXCR4/CXCR7-reactive neuroblasts, which migrate in close association with CXCL12 RGCs, layer-specific subsets of CXCL12 RGCs and astrocytes specifically contact the microvessel wall. Moreover, the CXCL12/CXCR4/CXCR7 system appears to be directly involved in microvessel growth, its members being differentially expressed in angiogenically activated microvessels and vascular sprouts.

Keywords

Cortical Plate CXCL12 Expression Chemokine Receptor CXCR4 Chemokine CXCL12 Human Cerebral Cortex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

M.V.C. Pragnell is gratefully acknowledged for language help.

Details of funding

This work has been funded by grants from Fondazione Cassa di Risparmio di Puglia (FCRP 2010) and from the Italian Ministry of the University and Scientific Research (MIUR), project PRIN 2008.

The authors confirm independence from the sponsors; the content of the article has not been influenced by the sponsors.

Conflict of interest

None.

Supplementary material

10545_2012_9574_Fig10_ESM.jpg (387 kb)
Supplementary material 1

A gallery of steps in human forebrain vascularization, from a preplate stage (A) to the cortex pre-lamination stage at midgestation (I). Microvessels are revealed by immunostaining for vessel basal lamina components, collagen IV (A, B, D, E, G, I) and fibronectin (C, F, H). The first penetrating microvessels (A, D, G), a capillary loop (B), radial stem vessels (C). E and F, details of stage G and C, respectively. Initial (H) and late (I) formation of H-shaped collaterals. MZ, marginal zone; CP, cortical plate; IZ, intermediate zone; SVZ, subventricular zone; VZ, ventricular zone. Immunolabelling with rabbit anti-collagen type IV (1:100 dilution; Acris Antibodies; Hiddenhausen, Germany) and rabbit anti-fibronectin (1:500 dilution; Millipore, Billerica, MA, USA), nuclear counterstaining with propidium iodide (Molecular Probes). (JPEG 386 kb)

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High resolution image (TIFF 31684 kb)
10545_2012_9574_Fig11_ESM.jpg (61 kb)
Supplementary material 2

A schematic outline of cerebral cortex, radial astrocytes depicted in Fig. 6. In the cortex, radial astrocytes appear strongly labelled by both GFAP and CXCL12, with complete overlapping of the two markers. In type 1 and 2 the nucleus is positioned on the top, close to the short, basal processes and to the origin of recurrent processes (arrowhead), while radial astrocytes type 3 and 4 have an upside-down position. (JPEG 60 kb)

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High resolution image (TIFF 9891 kb)
10545_2012_9574_MOESM3_ESM.avi (150 mb)
Supplementary material 3 A short series of single optical planes showing GFAP/CXCL12high RGC and astrocyte perivascular contacts (projection image in Fig. 5f). (AVI 153605 kb)
10545_2012_9574_Fig12_ESM.jpg (112 kb)
Supplementary material 4

Triple immunostaining with GFAP, CXCL12, and collagen type-IV. A cortex microvessel (asterisk) showing a CXCL12-reactive endothelial cell (arrow) and perivascular GFAP/CXCL12high astrocyte endfeet (arrowhead). (JPEG 111 kb)

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High resolution image (TIFF 8739 kb)

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Copyright information

© SSIEM and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Daniela Virgintino
    • 1
    • 6
  • Mariella Errede
    • 1
  • Marco Rizzi
    • 1
  • Francesco Girolamo
    • 1
  • Maurizio Strippoli
    • 1
  • Thomas Wälchli
    • 4
    • 5
  • David Robertson
    • 2
  • Karl Frei
    • 3
  • Luisa Roncali
    • 1
  1. 1.Department of Basic Medical Sciences, Neurosciences, Sensory Organs-Human Anatomy and Histology UnitUniversity of Bari School of MedicineBariItaly
  2. 2.Breakthrough Breast Cancer Research CentreThe Institute of Cancer ResearchLondonUK
  3. 3.Department of NeurosurgeryUniversity Hospital ZurichZurichSwitzerland
  4. 4.Brain Research InstituteUniversity of ZurichZurichSwitzerland
  5. 5.Department of Health Sciences and TechnologySwiss Federal Institute of Technology (ETH) ZurichZurichSwitzerland
  6. 6.Department of Basic Medical Sciences, Neurosciences, Sensory Organs-Human Anatomy and Histology UnitUniversity of Bari ‘Aldo Moro’BariItaly

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