One-step microchip for DNA fluorescent labeling
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In this study, we propose a microchip that is sequentially capable of fluorescently staining and washing DNAs. The main advantage of this microchip is that it allows for one-step preparation of small amounts of solution without degrading microscopic bio-objects such as the DNAs, cells, and biomolecules to be stained. The microchip consists of two inlets, the main channel, staining zone, washing zone, and one outlet, and was processed using a femtosecond laser system. High molecular transport of rhodamine B to deionized water was observed in the performance test of the microchip. Results revealed that the one-step procedure of on-chip DNA staining and washing was excellent compared to the conventional staining method. The one-step preparation of stained and washed DNAs through the microchip will be useful for preparing small volumes of experimental samples.
KeywordsStaining DNA Microchip Femtosecond laser Diffusion
This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017R1A4A1015681, NRF-2018R1D1A3B07047434, and 2019R1I1A3A01060695).
Conceptualization, Jinmu Jung and Jonghyun Oh; Data curation, Yeongseok Jang and Hojun Shin; Formal analysis, Yeongseok Jang and Hojun Shin; Investigation, Yeongseok Jang and Hojun Shin; Project administration, Jinmu Jung and Jonghyun Oh; Supervision, Jinmu Jung and Jonghyun Oh; Writing—original draft, Yeongseok Jang and Hojun Shin; Writing—review & editing, Jinmu Jung and Jonghyun Oh.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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