The evaluation of E. faecalis colonies dissolution ability of sodium hypochlorite in microenvironment by a novel device

  • Xiaoqiang Sun
  • Shaorong Li
  • Shujing Wang
  • Chunxiong LuoEmail author
  • Benxiang HouEmail author


Enterococcus faecalis(E. faecalis) is a common microorganism could be isolated from the infected canals, especially in the case of refractory apical periodontitis. Due to its ability to invade the dentinal tubules and highly resistant to antimicrobial strategies, the thorough debridement of E.faecalis is hard to achieve. And that may be one of the reasons to cause reinfection and therapeutic failure. According to the anatomy of dentinal tubules published before and the results of our team previous work, we designed six types of microtubes with different sizes. By using the method of centrifugation and incubation, a standard infected model mimicking dentinal tubules was established. Sodium hypochlorite (NaClO) is the most popular irrigant applied in root canal treatment. We used three different concentrations with four distinct irrigation duration to observe the antibacterial process of E. faecalis colonies within microtubes dynamically. We concluded that the role of NaClO in the microtubes is concentration dependent and duration dependent. And the interpretation of the results has a certain reference value for clinicians.


Microtube Dentinal tubules Enterococcus faecalis Sodium hypochlorite Dissolution 



This study was supported by the NSF of China (11674010, 11434001, 81170952) and Science and Technology Project of Dongcheng District, Beijing 2017-3-004.


  1. A. Ardizzoni, L. Generali, E. Righi, et al., Differential efficacy of endodontic obturation procedures: An ex vivo study. Odontology 102(2), 223–231 (2014)CrossRefGoogle Scholar
  2. V.B. Berber, B.P. Gomes, N.T. Sena, et al., Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing enterococcus faecalis within root canals and dentinal tubules. Int. Endod. J. 39(1), 10–17 (2006)CrossRefGoogle Scholar
  3. G. Bryce, D. O'Donnell, D. Ready, et al., Contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. J. Endod. 35(9), 1243–1248 (2009)CrossRefGoogle Scholar
  4. N.P. Chau, N.H. Chung, J.G. Jeon, Relationships between the antibacterial activity of sodium hypochlorite and treatment time and biofilm age in early enterococcus faecalis biofilms. Int. Endod. J. 48(8), 782–789 (2015)CrossRefGoogle Scholar
  5. P. Chivatxaranukul, S.G. Dashper, H.H. Messer, Dentinal tubule invasion and adherence by enterococcus faecalis. Int. Endod. J. 41(10), 873–882 (2008)CrossRefGoogle Scholar
  6. J.K. Cullen, J.A. Wealleans, T.C. Kirkpatrick, et al., The effect of 8.25% sodium hypochlorite on dental pulp dissolution and dentin flexural strength and modulus. J. Endod. 41(6), 920–924 (2015)CrossRefGoogle Scholar
  7. T. Du, Z. Wang, Y. Shen, et al., Effect of long-term exposure to endodontic disinfecting solutions on young and old enterococcus faecalis biofilms in dentin canals. J. Endod. 40(4), 509–514 (2014)CrossRefGoogle Scholar
  8. S.A. Farook, V. Shah, D. Lenouvel, et al., Guidelines for management of sodium hypochlorite extrusion injuries. Br. Dent. J. 217(12), 679–684 (2014)CrossRefGoogle Scholar
  9. D. Figdor, J.K. Davies, G. Sundqvist, Starvation survival, growth and recovery of enterococcus faecalis in human serum. Oral Microbiol. Immunol. 18(4), 234–239 (2003)CrossRefGoogle Scholar
  10. J.M. Guerreiro-Tanomaru, N.B. de Faria-Junior, M.A. Duarte, et al., Comparative analysis of enterococcus faecalis biofilm formation on different substrates. J. Endod. 39(3), 346–350 (2013)CrossRefGoogle Scholar
  11. T.A. Krause, F.R. Liewehr, C.L. Hahn, The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline, and citric acid on enterococcus faecalis. J. Endod. 33(1), 28–30 (2007)CrossRefGoogle Scholar
  12. R.M. Love, Invasion of dentinal tubules by root canal bacteria. Endod. Topics. 9(1), 52–65 (2010)CrossRefGoogle Scholar
  13. J. Ma, Z. Wang, Y. Shen, M. Haapasalo, A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy. J. Endod. 37(10), 1380–1385 (2011)CrossRefGoogle Scholar
  14. S.A. Mohmmed, M.E. Vianna, M.R. Penny, et al., The effect of sodium hypochlorite concentration and irrigation needle extension on biofilm removal from a simulated root canal model. Aust. Endod. J. 43(3), 102–109 (2017a)CrossRefGoogle Scholar
  15. S.A. Mohmmed, M.E. Vianna, M.R. Penny, et al., Confocal laser scanning, scanning electron, and transmission electron microscopy investigation of enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model. Microbiology 6(4) (2017b)Google Scholar
  16. W.R. Moorer, P.R. Wesselink, Factors promoting the tissue dissolving capability of sodium hypochlorite. Int. Endod. J. 15(4), 187–196 (1982)CrossRefGoogle Scholar
  17. M. Nourzadeh, A. Amini, F. Fakoor, et al., Comparative antimicrobial efficacy of Eucalyptus Galbie and Myrtus Communis L. extracts, chlorhexidine and sodium hypochlorite against enterococcus Faecalis. Iran. Endod. J. 12(2), 205–210 (2017)Google Scholar
  18. F. Palazzi, A. Blasi, Z. Mohammadi, et al., Penetration of sodium hypochlorite modified with surfactants into root canal dentin. Braz. Dent. J. 27(2), 208–216 (2016)CrossRefGoogle Scholar
  19. E.T. Pinheiro, B.P. Gomes, C.C. Ferraz, et al., Microorganisms from canals of root-filled teeth with periapical lesions. Int. Endod. J. 36(1), 1–11 (2003)CrossRefGoogle Scholar
  20. S. Ran, J. Wang, W. Jiang, et al., Assessment of dentinal tubule invasion capacity of enterococcus faecalis under stress conditions ex vivo. Int. Endod. J. 48(4), 362–372 (2015)CrossRefGoogle Scholar
  21. B. Retamozo, S. Shabahang, N. Johnson, et al., Minimum contact time and concentration of sodium hypochlorite required to eliminate enterococcus faecalis. J. Endod. 36(3), 520–523 (2010)CrossRefGoogle Scholar
  22. G. Rossi-Fedele, J.A. Figueiredo, L. Steier, et al., Evaluation of the antimicrobial effect of super-oxidized water (Sterilox(R)) and sodium hypochlorite against enterococcus faecalis in a bovine root canal model. J. Appl. Oral Sci. 18(5), 498–502 (2010)CrossRefGoogle Scholar
  23. M.M. Shirazi, O. Abouali, H. Emdad, et al., Numerical and analytical investigation of irrigant penetration into dentinal microtubules. Comput. Biol. Med. 89, 1–17 (2017)CrossRefGoogle Scholar
  24. B.W. Sigusch, S. Kranz, S. Klein, et al., Colonization of enterococcus faecalis in a new SiO/SiO(2)-microtube in vitro model system as a function of tubule diameter. Dent. Mater. 30(6), 661–668 (2014)CrossRefGoogle Scholar
  25. T.P. Sim, J.C. Knowles, Y.L. Ng, et al., Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain. Int. Endod. J. 34(2), 120–132 (2001)CrossRefGoogle Scholar
  26. J.J. Siqueira, I.N. Rocas, Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 97(1), 85–94 (2004)CrossRefGoogle Scholar
  27. S. Stojicic, S. Zivkovic, W. Qian, et al., Tissue dissolution by sodium hypochlorite: Effect of concentration, temperature, agitation, and surfactant. J. Endod. 36(9), 1558–1562 (2010)CrossRefGoogle Scholar
  28. S. Stojicic, Y. Shen, M. Haapasalo, Effect of the source of biofilm bacteria, level of biofilm maturation, and type of disinfecting agent on the susceptibility of biofilm bacteria to antibacterial agents. J. Endod. 39(4), 473–477 (2013)CrossRefGoogle Scholar
  29. X. Sun, S. Wang, Y. Yang, et al., Study of invasion and colonization of E. faecalis in microtubes by a novel device. Biomed. MIcrodevices 18(5), 82 (2016)CrossRefGoogle Scholar
  30. S. Taschieri, F.M. Del, L. Samaranayake, et al., Microbial invasion of dentinal tubules: A literature review and a new perspective. J. Investig. Clin. Dent. 5(3), 163–170 (2014)CrossRefGoogle Scholar
  31. D.T. Wong, G.S. Cheung, Extension of bactericidal effect of sodium hypochlorite into dentinal tubules. J. Endod. 40(6), 825–829 (2014)CrossRefGoogle Scholar
  32. M. Zehnder, Root canal irrigants. J. Endod. 32(5), 389–398 (2006)CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Department of Endodontics, School of StomatologyCapital Medical UniversityBeijingChina
  2. 2.The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of PhysicsPeking UniversityBeijingChina
  3. 3.Center for Quantitative Biology, Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingChina

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