Design and development of a microarray processing station (MPS) for automated miniaturized immunoassays
Here we describe the design and evaluation of a fluidic device for the automatic processing of microarrays, called microarray processing station or MPS. The microarray processing station once installed on a commercial microarrayer allows automating the washing, and drying steps, which are often performed manually. The substrate where the assay occurs remains on place during the microarray printing, incubation and processing steps, therefore the addressing of nL volumes of the distinct immunoassay reagents such as capture and detection antibodies and samples can be performed on the same coordinate of the substrate with a perfect alignment without requiring any additional mechanical or optical re-alignment methods. This allows the performance of independent immunoassays in a single microarray spot.
KeywordsAutomation Microarrays High-throughput screening Immunoassays Customization
This work was supported by Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain. The Nanobioengineering group at the Institute for Bioengineering of Catalonia (IBEC) receives support from the Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya (No. 2014 SGR 1442). In addition, the research received support from the Spanish Ministry of Economy and Competitiveness, OLIGOCODES (MAT2012-38573-C02), and the Fundación Botín, Santander, Spain. M. Pla-Roca acknowledges a Beatriu de Pinos fellowship (2010 BP-B00180) from the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR).
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