Biomedical Microdevices

, 18:64 | Cite as

Design and development of a microarray processing station (MPS) for automated miniaturized immunoassays

  • Mateu Pla-Roca
  • Gizem Altay
  • Xavier Giralt
  • Alícia Casals
  • Josep Samitier
Article

Abstract

Here we describe the design and evaluation of a fluidic device for the automatic processing of microarrays, called microarray processing station or MPS. The microarray processing station once installed on a commercial microarrayer allows automating the washing, and drying steps, which are often performed manually. The substrate where the assay occurs remains on place during the microarray printing, incubation and processing steps, therefore the addressing of nL volumes of the distinct immunoassay reagents such as capture and detection antibodies and samples can be performed on the same coordinate of the substrate with a perfect alignment without requiring any additional mechanical or optical re-alignment methods. This allows the performance of independent immunoassays in a single microarray spot.

Keywords

Automation Microarrays High-throughput screening Immunoassays Customization 

Notes

Acknowledgments

This work was supported by Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain. The Nanobioengineering group at the Institute for Bioengineering of Catalonia (IBEC) receives support from the Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya (No. 2014 SGR 1442). In addition, the research received support from the Spanish Ministry of Economy and Competitiveness, OLIGOCODES (MAT2012-38573-C02), and the Fundación Botín, Santander, Spain. M. Pla-Roca acknowledges a Beatriu de Pinos fellowship (2010 BP-B00180) from the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.s

Human and animal rights

“This article does not contain any studies with human participants or animals performed by any of the authors.”

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Mateu Pla-Roca
    • 1
  • Gizem Altay
    • 1
  • Xavier Giralt
    • 2
  • Alícia Casals
    • 2
    • 4
  • Josep Samitier
    • 1
    • 3
    • 5
  1. 1.Nanobioengineering LaboratoryInstitute for Bioengineering of Catalonia (IBEC)BarcelonaSpain
  2. 2.Robotics LaboratoryInstitute for Bioengineering of Catalonia (IBEC)BarcelonaSpain
  3. 3.The Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)ZaragozaSpain
  4. 4.Center of Research in Biomedical EngineeringUniversitat Politècnica de CatalunyaBarcelonaSpain
  5. 5.Department of Engineering: ElectronicsUniversity of Barcelona (UB)BarcelonaSpain

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