Acoustophoretic removal of proteins from blood components
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This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.
KeywordsAcoustophoresis Blood component preparation IgA deficiency
We gratefully thank Sandra Pettersson at the Department of Transfusion Medicine, Skånes University Hospital, Lund, Sweden for providing the RBC concentrate samples. Financial support for this work has been provided by The Swedish Governmental Agency for Innovation Systems, VINNOVA, the project “CellCARE” (grant no. 2009–00236) and the Swedish Research Council FORMAS through the Strong Research Environments project “BioBridges” (project no. 221-2011-1692).
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