Biomedical Microdevices

, 17:95 | Cite as

Acoustophoretic removal of proteins from blood components

  • Maria Tenje
  • Maria N. Lundgren
  • Ann-Margret Swärd-Nilsson
  • Jens Kjeldsen-Kragh
  • Lena Lyxe
  • Andreas Lenshof
Article

Abstract

This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

Keywords

Acoustophoresis Blood component preparation IgA deficiency 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Maria Tenje
    • 1
    • 2
  • Maria N. Lundgren
    • 3
  • Ann-Margret Swärd-Nilsson
    • 3
  • Jens Kjeldsen-Kragh
    • 3
  • Lena Lyxe
    • 4
  • Andreas Lenshof
    • 1
  1. 1.Department of Biomedical EngineeringLund UniversityLundSweden
  2. 2.Department of Engineering Sciences, Science for Life LaboratoryUppsala UniversityUppsalaSweden
  3. 3.Department of Clinical Immunology and Transfusion MedicineSkåne University HospitalLundSweden
  4. 4.Sahlgrenska University HospitalGöteborgSweden

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