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Biomedical Microdevices

, Volume 16, Issue 4, pp 501–508 | Cite as

Microtubule shuttles on kinesin-coated glass micro-wire tracks

  • Kyongwan Kim
  • Andrew L. Liao
  • Aurélien Sikora
  • Daniel Oliveira
  • Hikaru Nakazawa
  • Mitsuo Umetsu
  • Izumi Kumagai
  • Tadafumi Adschiri
  • Wonmuk Hwang
  • Winfried TeizerEmail author
Article

Abstract

Gliding of microtubule filaments on surfaces coated with the motor protein kinesin has potential applications for nano-scale devices. The ability to guide the gliding direction in three dimensions allows the fabrication of tracks of arbitrary geometry in space. Here, we achieve this by using kinesin-coated glass wires of micrometer diameter range. Unlike previous methods in which the guiding tracks are fixed on flat two-dimensional surfaces, the flexibility of glass wires in shape and size facilitates building in-vitro devices that have deformable tracks.

Keywords

Microtubule Kinesin Molecular motility Glass micro-wire Lab-on-a-chip Molecular delivery 

Notes

Acknowledgments

We gratefully acknowledge support from the World Premier International Research Center Initiative (WPI), MEXT, Japan. We would like to thank Dr. Hideaki Sanada for generously providing the kinesin plasmid.

Supplementary material

SM01

A glass wire decorated by quantum dots in a buffer solution (Images ~100 μm × 100 μm in size). (MPG 2056 kb)

SM02–SM11

Microtubules gliding on the kinesin coated glass wire. The focus was adjusted occasionally in each movie (Images ~50 μm × 50 μm in size, actual elapsed time 4 min 32 s). (MPG 366 kb)

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SM12

Microtubules gliding on the kinesin coated surface of the coverslip (Images ~50 μm × 50 μm in size, actual elapsed time 4 min 32 s). (MPG 1438 kb)

SM13 and SM14

Microtubules gliding towards two separate chip surfaces along the kinesin coated glass wire bridges (Images ~50 μm × 50 μm in size, time tagged on the images (min:sec)). (MPG 149 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Kyongwan Kim
    • 1
  • Andrew L. Liao
    • 1
    • 2
  • Aurélien Sikora
    • 1
  • Daniel Oliveira
    • 1
  • Hikaru Nakazawa
    • 3
  • Mitsuo Umetsu
    • 1
    • 3
  • Izumi Kumagai
    • 3
  • Tadafumi Adschiri
    • 1
  • Wonmuk Hwang
    • 2
    • 4
    • 5
  • Winfried Teizer
    • 1
    • 2
    • 6
    Email author
  1. 1.WPI-Advanced Institute for Materials Research (AIMR)Tohoku UniversitySendaiJapan
  2. 2.Materials Science and EngineeringTexas A&M UniversityCollege StationUSA
  3. 3.Department of Biomolecular Engineering, Graduate School of EngineeringTohoku UniversitySendaiJapan
  4. 4.Department of Biomedical EngineeringTexas A&M UniversityCollege StationUSA
  5. 5.School of Computational SciencesKorea Institute for Advanced StudySeoulSouth Korea
  6. 6.Department of Physics and AstronomyTexas A&M UniversityCollege StationUSA

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