Biomedical Microdevices

, Volume 16, Issue 2, pp 245–253 | Cite as

Oil–water biphasic parallel flow for the precise patterning of metals and cells

  • Xuan Mu
  • Qionglin LiangEmail author
  • Jun Zhou
  • Kangning Ren
  • Ping Hu
  • Yiming Wang
  • Zhi ZhengEmail author
  • Guoan LuoEmail author


Fluidic patterning is a convenient and versatile tool for the patterning of materials, cells and microstructures on surface and in microchannels. However, its performance is usually limited by transverse diffusion between fluid streams. It would blur the boundary and deteriorate the precision of patterns. In this paper, we adopted geometric confinement to generate biphasic parallel flow that is constituted of oil and water. Since there is minimum transverse diffusion in biphasic parallel flow, the performance of fluid patterning is expected to be improved. The results show that the metal (Silver and Chromium) patterns have distinct boundary and well-controlled geometry in comparison with that by conventional laminar flow patterning. Furthermore, the high biocompatibility of oil phase (perfluorodecalin, PFD) enables the precise patterning of viable bacteria inside microchannels. Our work demonstrated a new route of using biphasic parallel flow to patterning, which would serve wide applications in prototyping and research settings.


Microfluidics Patterning Metal Bacteria Biphasic flow Perfluorodecalin 



This work was supported by National Science and Technology Major Project of China (2013ZX09507005), National Natural Science Foundation of China (21305162, 21235004 and 21175080), the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions (to Q. L.). The authors thank Prof. Xingyu Jiang in National Center of Nanoscience and Technology for providing fluorescence microscope, and Prof. Bo Zheng at Chinese University of HongKong, Prof. Bo Yao at Zhejiang University and Prof. Ho Cheung Shum at The University of HongKong for fruitful discussions.

Supplementary material

10544_2013_9828_MOESM1_ESM.doc (948 kb)
ESM 1 (DOC 948 kb)

Video (MPG 4022 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingPeople’s Republic of China
  2. 2.Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of ChemistryTsinghua UniversityBeijingPeople’s Republic of China
  3. 3.College of Chemical and Molecular EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China

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