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Superparamagnetic microrobots: fabrication by two-photon polymerization and biocompatibility

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Abstract

This work presents the fabrication and controlled actuation of swimming microrobots made of a magnetic polymer composite (MPC) consisting of 11-nm-diameter magnetite (Fe3O4) nanoparticles and photocurable resin (SU-8). Two-photon polymerization (TPP) is used to fabricate the magnetic microstructures. The material properties and the cytotoxicity of the MPC with different nanoparticle concentrations are characterized. The live/dead staining tests indicate that MPC samples with varied concentrations, up to 10 vol.%, have negligible cytotoxicity after 24 h incubation. Fabrication parameters of MPC with up to 4 vol.% were investigated. We demonstrate that the helical microdevices made of 2 vol.% MPC were capable of performing corkscrew motion in water applying weak uniform rotating magnetic fields.

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References

  1. R.A. Anderson, H.C. Berg, Bacteria swim by rotating their flagellar filaments. Nature 245, 380–382 (1973)

  2. M. Colombo, S. Carregal-Romero, M.F. Casula, L. Gutierrez, M.P. Morales, I.B. Bohm, J.T. Heverhagen, D. Prosperi, W.J. Parak, Biological applications of magnetic nanoparticles. Chem. Soc. Rev. 41, 4306–4334 (2012)

  3. A. Deepu, V.V.R. Sai, S. Mukherji, Simple surface modification techniques for immobilization of biomolecules on SU-8. J. Mater. Sci. 20, 25–28 (2009)

  4. D.L. Fan, Z.Z. Yin, R. Cheong, F.Q. Zhu, R.C. Cammarata, C.L. Chien, A. Levchenko, Subcellular-resolution delivery of a cytokine through precisely manipulated nanowires. Nat. Nanotechnol. 5, 545–551 (2010)

  5. E. Frances, J. Burdan, A. Cutino, K.E. Green, A new sustained delivery technology for posterior eye disease. Expert Opin. Drug Deliv. 5, 1039–1046 (2008)

  6. A. Ghosh, P. Fischer, Controlled propulsion of artificial magnetic nano-structured propellers. Nano Lett. 9, 2243–2245 (2009)

  7. M. Hagiwara, T. Kawahara, Y. Yamanishi, T. Masuda, L. Feng, F. Arai, On-chip magnetically actuated robot with ultrasonic vibration for single cell manipulations. Lab Chip 11, 2049–2054 (2011)

  8. J. Kim, H.S. Kim, N. Lee, T. Kim, H. Kim, T. Yu, I.C. Song, W.K. Moon, T. Hyeon, Multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance and fluorescence imaging and for drug delivery. Angew. Chem. Int. Ed. 47, 8438–8441 (2008)

  9. A.H. Lu, E.L. Salabas, F. Schuth, Magnetic nanoparticles: synthesis, protection, functionalization, and application. Angew. Chem. Int. Ed. 46, 1222–1244 (2007)

  10. S. Martel, M. Mohammadi, O. Felfoul, Z. Lu, P. Pouponneau, Flagellated magnetotactic bacteria as controlled MRI-trackable propulsion and steering systems for medical nanorobots operating in the human microvasculature. Int. J. Robot. Res. 28, 571–582 (2009)

  11. Y.F. Mei, A.A. Solovev, S. Sanchez, O.G. Schmidt, Rolled-up nanotech on polymers: from basic perception to self-propelled catalytic microengines. Chem. Soc. Rev. 40, 2109–2119 (2011)

  12. B.J. Nelson, I.K. Kaliakatsos, J.J. Abbott, Microrobots for minimally invasive medicine. Annu. Rev. Biomed. Eng. 12, 55–85 (2010)

  13. G.A. Ozin, I. Manners, S. Fournier-Bidoz, A. Arsenault, Dream Nanomachines. Adv. Mater. 17, 3011–3018 (2005)

  14. S.H. Park, D.Y. Yang, K.S. Lee, Two-photon stereolithography for realizing ultraprecise three-dimensional nano/microdevices. Laser Photonics Rev. 3, 1–11 (2009)

  15. C. Peters, O. Ergeneman, B. J. Nelson, C. Hierold, Pushing the limits of photo-curable SU-8-based superparamagnetic polymer composites, Proc. Int. Conf. on Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS) (Barcelona, 2013), pp 2676–2679

  16. K.E. Peyer, L. Zhang, B.J. Nelson, Bio-inspired magnetic swimming microrobots for biomedical applications. Nanoscale 5, 1259–1272 (2013a)

  17. K.E. Peyer, S. Tottori, F. Qiu, L. Zhang, B.J. Nelson, Magnetic helical micromachines. Chem. Eur. J. 19, 28–38 (2013b)

  18. E.M. Purcell, Life at low Reynolds number. Am. J. Phys. 45, 3–11 (1977)

  19. F.M. Qiu, L. Zhang, S. Tottori, K. Marquardt, K. Krawczyk, A. Franco-Obregon, B.J. Nelson, Bio-inspired microrobots: the first intimate contact with cells. Mater. Today 15, 463 (2012)

  20. S. Schuerle, S. Pane, E. Pellicer, J. Sort, M.D. Baro, B.J. Nelson, Helical and tubular lipid microstructures that are electroless-coated with CoNiReP for wireless magnetic manipulation. Small 8, 1498–1502 (2012)

  21. J.J. Shi, D. Ahmed, X. Mao, S.C.S. Lin, A. Lawit, T.J. Huang, Acoustic tweezers: patterning cells and microparticles using standing surface acoustic waves (SSAW). Lab Chip 9, 2890–2895 (2009)

  22. C.E. Sing, L. Schmid, M.F. Schneider, T. Franke, A. Alexander-Katz, Controlled surface-induced flows from the motion of self-assembled colloidal walkers. Proc. Natl. Acad. Sci. U. S. A. 107, 535–540 (2010)

  23. K.M. Sivaraman, C. Kellenberger, S. Pane, O. Ergeneman, T. Luhmann, N.A. Luechinger, H. Hall, W.J. Stark, B.J. Nelson, Porous polysulfone coatings for enhanced drug delivery. Biomed. Microdevices 14, 603–612 (2012)

  24. A.A. Solovev, W. Xi, D.H. Gracias, S.M. Harazim, C. Deneke, S. Sanchez, O.G. Schmidt, Self-propelled nanotools. ACS Nano 6, 1751–1756 (2012)

  25. M. Suter, O. Ergeneman, J. Zurcher, C. Moitzi, S. Pane, T. Rudin, S.E. Pratsinis, B.J. Nelson, C. Hierold, A photopatternable superparamagnetic nanocomposite: material characterization and fabrication of microstructures. Sensors Actuat. B Chem. 156, 433–443 (2011a)

  26. M. Suter, O. Ergeneman, J. Zurcher, S. Schmid, A. Camenzind, B.J. Nelson, C. Hierold, Superparamagnetic photocurable nanocomposite for the fabrication of microcantilevers. J. Micromech. Microeng. 21, 025023 (2011b)

  27. M. Suter, Photopatternable superparamagnetic nanocomposite for the fabrication of microstructures, Ph.D. dissertation, ETH Zurich, (2012)

  28. Y. Tian, Y.L. Zhang, J.F. Ku, Y. He, B.B. Xu, Q.D. Chen, H. Xia, H.B. Sun, High performance magnetically controllable microturbines. Lab Chip 10, 2902–2905 (2010)

  29. S. Tottori, L. Zhang, F.M. Qiu, K.K. Krawczyk, A. Franco-Obregon, B.J. Nelson, Magnetic helical micromachines: fabrication, controlled swimming, and cargo transport. Adv. Mater. 24, 811–816 (2012)

  30. J. Wang, Cargo-towing synthetic nanomachines: towards active transport in microchip devices. Lab Chip 12, 1944–1950 (2012)

  31. H. Xia, J.A. Wang, Y. Tian, Q.D. Chen, X.B. Du, Y.L. Zhang, Y. He, H.B. Sun, Ferrofluids for fabrication of remotely controllable micro-nanomachines by two-photon polymerization. Adv. Mater. 22, 3204–3207 (2010)

  32. L. Zhang, J.J. Abbott, L.X. Dong, B.E. Kratochvil, D. Bell, B.J. Nelson, Artificial bacterial flagella: fabrication and magnetic control. Appl. Phys. Lett. 94, 064107 (2009a)

  33. L. Zhang, J.J. Abbott, L. Dong, K.E. Peyer, B.E. Kratochvil, H. Zhang, C. Bergeles, B.J. Nelson, Characterizing the swimming properties of artificial bacterial flagella. Nano Lett. 9, 3663–3667 (2009b)

  34. Y.L. Zhang, Q.D. Chen, H. Xia, H.B. Sun, Designable 3D nanofabrication by femtosecond laser direct writing. Nano Today 5, 435–448 (2010a)

  35. L. Zhang, K.E. Peyer, B.J. Nelson, Artificial bacterial flagella for micromanipulation. Lab Chip 10, 2203–2215 (2010b)

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Acknowledgments

The authors thank Eszter Barthazy and Elisabeth Müller (EMEZ at ETH Zurich) for the TEM-images, the technical support from the FIRST lab at ETH Zurich. Funding for this research is provided by Swiss National Science Foundation (SNSF), project number 130069 and the ETH Zurich (TH-28 06–3).

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Correspondence to Li Zhang.

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Suter, M., Zhang, L., Siringil, E.C. et al. Superparamagnetic microrobots: fabrication by two-photon polymerization and biocompatibility. Biomed Microdevices 15, 997–1003 (2013). https://doi.org/10.1007/s10544-013-9791-7

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Keywords

  • Superparamagnetic nanocomposite
  • Microrobots
  • Magnetic actuation
  • Cytotoxicity